Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ETA10_RS13040 Genome accession   NZ_CP035397
Coordinates   2420265..2420675 (-) Length   136 a.a.
NCBI ID   WP_129110531.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103773     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2415265..2425675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETA10_RS13010 (ETA10_13010) - 2415352..2415492 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ETA10_RS13015 (ETA10_13015) yqeF 2415798..2416529 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ETA10_RS13020 (ETA10_13020) cwlH 2416781..2417533 (-) 753 WP_015251672.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ETA10_RS13025 (ETA10_13025) yqeD 2417720..2418346 (+) 627 WP_015251671.1 TVP38/TMEM64 family protein -
  ETA10_RS13030 (ETA10_13030) gnd 2418365..2419258 (-) 894 WP_015251670.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ETA10_RS13035 (ETA10_13035) yqeB 2419510..2420232 (+) 723 WP_010886572.1 hypothetical protein -
  ETA10_RS13040 (ETA10_13040) nucA/comI 2420265..2420675 (-) 411 WP_129110531.1 sporulation-specific Dnase NucB Machinery gene
  ETA10_RS13045 (ETA10_13045) - 2420871..2421218 (+) 348 Protein_2512 sigma-70 family RNA polymerase sigma factor -
  ETA10_RS13050 (ETA10_13050) spoIVCA 2421249..2422709 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  ETA10_RS22550 (ETA10_13055) - 2422667..2422845 (-) 179 Protein_2514 hypothetical protein -
  ETA10_RS13060 (ETA10_13060) arsC 2423200..2423619 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  ETA10_RS13065 (ETA10_13065) acr3 2423631..2424671 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  ETA10_RS13070 (ETA10_13070) arsK 2424694..2425134 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  ETA10_RS13075 (ETA10_13075) arsR 2425193..2425510 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14939.96 Da        Isoelectric Point: 5.1853

>NTDB_id=299214 ETA10_RS13040 WP_129110531.1 2420265..2420675(-) (nucA/comI) [Bacillus subtilis strain SRCM103773]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIKDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=299214 ETA10_RS13040 WP_129110531.1 2420265..2420675(-) (nucA/comI) [Bacillus subtilis strain SRCM103773]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAAGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.478

84.559

0.537