Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   DOU34_RS10610 Genome accession   NZ_CP030097
Coordinates   2206900..2207700 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2201900..2212700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS10580 - 2202440..2203072 (+) 633 WP_007408365.1 helix-turn-helix domain-containing protein -
  DOU34_RS20920 - 2203369..2203506 (+) 138 WP_162839613.1 hypothetical protein -
  DOU34_RS10585 - 2203600..2204718 (+) 1119 WP_032863662.1 NADH-dependent flavin oxidoreductase -
  DOU34_RS10590 - 2204845..2205057 (+) 213 WP_007408362.1 spore germination protein -
  DOU34_RS10595 - 2205182..2205394 (+) 213 WP_003153181.1 spore germination protein -
  DOU34_RS10600 alr 2205570..2206751 (+) 1182 WP_007408361.1 alanine racemase -
  DOU34_RS10610 spo0A 2206900..2207700 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  DOU34_RS10615 spoIVB 2207981..2209261 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  DOU34_RS10620 recN 2209425..2211155 (-) 1731 WP_061046623.1 DNA repair protein RecN Machinery gene
  DOU34_RS10625 argR 2211187..2211636 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  DOU34_RS10630 - 2211733..2212578 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=299116 DOU34_RS10610 WP_003153177.1 2206900..2207700(-) (spo0A) [Bacillus amyloliquefaciens strain SH-B74]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=299116 DOU34_RS10610 WP_003153177.1 2206900..2207700(-) (spo0A) [Bacillus amyloliquefaciens strain SH-B74]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCTGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAATTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment