Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DOU34_RS00375 Genome accession   NZ_CP030097
Coordinates   78786..79223 (-) Length   145 a.a.
NCBI ID   WP_032867344.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 73786..84223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS00350 - 74806..75942 (+) 1137 WP_007409359.1 zinc-dependent alcohol dehydrogenase -
  DOU34_RS00355 - 75957..76391 (+) 435 WP_007409358.1 RDD family protein -
  DOU34_RS00360 - 76463..76786 (+) 324 WP_007409357.1 YckD family protein -
  DOU34_RS00365 - 76890..78326 (+) 1437 WP_007409356.1 family 1 glycosylhydrolase -
  DOU34_RS00370 nin/comJ 78367..78765 (-) 399 WP_015239128.1 competence protein ComJ Regulator
  DOU34_RS00375 nucA/comI 78786..79223 (-) 438 WP_032867344.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DOU34_RS00380 hxlB 79581..80138 (-) 558 WP_015239129.1 6-phospho-3-hexuloisomerase -
  DOU34_RS00385 hxlA 80135..80770 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  DOU34_RS00390 - 81002..81364 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16094.41 Da        Isoelectric Point: 9.0073

>NTDB_id=299086 DOU34_RS00375 WP_032867344.1 78786..79223(-) (nucA/comI) [Bacillus amyloliquefaciens strain SH-B74]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSHYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=299086 DOU34_RS00375 WP_032867344.1 78786..79223(-) (nucA/comI) [Bacillus amyloliquefaciens strain SH-B74]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTCATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment