Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   DOU32_RS11450 Genome accession   NZ_CP030045
Coordinates   2237457..2239268 (-) Length   603 a.a.
NCBI ID   WP_002356480.1    Uniprot ID   -
Organism   Enterococcus faecalis strain C54     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2232457..2244268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU32_RS11425 (DOU32_11450) - 2233282..2234079 (-) 798 WP_002356486.1 NAD kinase -
  DOU32_RS11430 (DOU32_11455) - 2234092..2234766 (-) 675 WP_002356484.1 GTP pyrophosphokinase -
  DOU32_RS11435 (DOU32_11460) - 2234963..2235541 (+) 579 WP_002356483.1 CYTH domain-containing protein -
  DOU32_RS11440 (DOU32_11465) - 2235605..2236264 (+) 660 WP_002359562.1 ClpXP adapter SpxH family protein -
  DOU32_RS11445 (DOU32_11470) - 2236547..2237266 (-) 720 WP_002356481.1 helix-turn-helix transcriptional regulator -
  DOU32_RS11450 (DOU32_11475) pepF 2237457..2239268 (-) 1812 WP_002356480.1 oligoendopeptidase F Regulator
  DOU32_RS11455 (DOU32_11480) - 2239316..2240494 (-) 1179 WP_002356479.1 competence protein CoiA -
  DOU32_RS11460 (DOU32_11485) - 2240583..2241245 (-) 663 WP_002389051.1 adaptor protein MecA -
  DOU32_RS11465 (DOU32_11490) spxA 2241588..2241986 (-) 399 WP_002356477.1 transcriptional regulator SpxA -
  DOU32_RS11470 (DOU32_11495) trpS 2242346..2243356 (+) 1011 WP_002356476.1 tryptophan--tRNA ligase -

Sequence

Protein


Download         Length: 603 a.a.        Molecular weight: 68835.12 Da        Isoelectric Point: 4.7145

>NTDB_id=298731 DOU32_RS11450 WP_002356480.1 2237457..2239268(-) (pepF) [Enterococcus faecalis strain C54]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLTEELNQAESFKGTLGNGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide


Download         Length: 1812 bp        

>NTDB_id=298731 DOU32_RS11450 WP_002356480.1 2237457..2239268(-) (pepF) [Enterococcus faecalis strain C54]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTAACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGACAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGAACCTTAGGGA
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATAGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGATCCAGAGGTTTTAACATTAAGTGATGAACAAATTTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGGGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAATTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGGTTGTACAAAGTGTACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGGGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGTGGCGCATATTCTTCAGGAGCTTATGACACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACCCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAACTACCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

54.637

98.342

0.537

Multiple sequence alignment