Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   DOU31_RS02995 Genome accession   NZ_CP030042
Coordinates   597805..599616 (+) Length   603 a.a.
NCBI ID   WP_002356480.1    Uniprot ID   -
Organism   Enterococcus faecalis strain C25     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 592805..604616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU31_RS02975 (DOU31_02970) trpS 593718..594728 (-) 1011 WP_010717110.1 tryptophan--tRNA ligase -
  DOU31_RS02980 (DOU31_02975) spxA 595088..595486 (+) 399 WP_002356477.1 transcriptional regulator SpxA -
  DOU31_RS02985 (DOU31_02980) - 595829..596491 (+) 663 WP_002356478.1 adaptor protein MecA -
  DOU31_RS02990 (DOU31_02985) - 596579..597757 (+) 1179 WP_002372272.1 competence protein CoiA -
  DOU31_RS02995 (DOU31_02990) pepF 597805..599616 (+) 1812 WP_002356480.1 oligoendopeptidase F Regulator
  DOU31_RS03000 (DOU31_02995) - 599807..600526 (+) 720 WP_002356481.1 helix-turn-helix transcriptional regulator -
  DOU31_RS03005 (DOU31_03000) - 600809..601468 (-) 660 WP_002356482.1 ClpXP adapter SpxH family protein -
  DOU31_RS03010 (DOU31_03005) - 601532..602110 (-) 579 WP_002356483.1 CYTH domain-containing protein -
  DOU31_RS03015 (DOU31_03010) - 602307..602981 (+) 675 WP_002356484.1 GTP pyrophosphokinase -
  DOU31_RS03020 (DOU31_03015) - 602994..603791 (+) 798 WP_002356486.1 NAD kinase -

Sequence

Protein


Download         Length: 603 a.a.        Molecular weight: 68835.12 Da        Isoelectric Point: 4.7145

>NTDB_id=298675 DOU31_RS02995 WP_002356480.1 597805..599616(+) (pepF) [Enterococcus faecalis strain C25]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLTEELNQAESFKGTLGNGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide


Download         Length: 1812 bp        

>NTDB_id=298675 DOU31_RS02995 WP_002356480.1 597805..599616(+) (pepF) [Enterococcus faecalis strain C25]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTAACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGACAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGAACCTTAGGGA
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATAGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGATCCAGAGGTTTTAACATTAAGTGATGAACAAATTTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAATTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGGTTGTACAAAGTGTACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGGGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACGAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAACTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

54.637

98.342

0.537

Multiple sequence alignment