Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   ES669_RS23370 Genome accession   NZ_CP035366
Coordinates   4533553..4534221 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O157:H7 strain C1-057     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4528553..4539221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES669_RS23340 (ES669_23340) rfaQ 4528731..4529789 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  ES669_RS23345 (ES669_23345) waaA 4530202..4531479 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  ES669_RS23350 (ES669_23350) coaD 4531487..4531966 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  ES669_RS23355 (ES669_23355) mutM 4532005..4532814 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  ES669_RS23360 (ES669_23360) rpmG 4532912..4533079 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  ES669_RS23365 (ES669_23365) rpmB 4533100..4533336 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  ES669_RS23370 (ES669_23370) radC 4533553..4534221 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  ES669_RS23375 (ES669_23375) coaBC 4534393..4535613 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  ES669_RS23380 (ES669_23380) dut 4535591..4536049 (+) 459 WP_000976070.1 dUTP diphosphatase -
  ES669_RS23385 (ES669_23385) slmA 4536156..4536752 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  ES669_RS23390 (ES669_23390) pyrE 4536789..4537430 (-) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  ES669_RS23395 (ES669_23395) rph 4537496..4538212 (-) 717 WP_001247093.1 ribonuclease PH -
  ES669_RS23400 (ES669_23400) yicC 4538339..4539202 (+) 864 WP_000621341.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=298337 ES669_RS23370 WP_001297375.1 4533553..4534221(-) (radC) [Escherichia coli O157:H7 strain C1-057]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=298337 ES669_RS23370 WP_001297375.1 4533553..4534221(-) (radC) [Escherichia coli O157:H7 strain C1-057]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment