Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DN066_RS03120 Genome accession   NZ_CP030007
Coordinates   630647..632080 (-) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. xiangfangensis strain Pb204     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 625647..637080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN066_RS03090 (DN066_03090) elbB 627179..627832 (+) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -
  DN066_RS03095 (DN066_03095) mtgA 627829..628554 (+) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  DN066_RS03100 (DN066_03100) npr 628594..628866 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  DN066_RS03105 (DN066_03105) rapZ 628863..629717 (-) 855 WP_003861870.1 RNase adapter RapZ -
  DN066_RS03110 (DN066_03110) ptsN 629763..630254 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DN066_RS03115 (DN066_03115) hpf 630337..630624 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  DN066_RS03120 (DN066_03120) rpoN/rpoN1 630647..632080 (-) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  DN066_RS03125 (DN066_03125) lptB 632128..632853 (-) 726 WP_023304493.1 LPS export ABC transporter ATP-binding protein -
  DN066_RS03130 (DN066_03130) lptA 632860..633414 (-) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  DN066_RS03135 (DN066_03135) lptC 633383..633958 (-) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  DN066_RS03140 (DN066_03140) kdsC 633955..634521 (-) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  DN066_RS03145 (DN066_03145) kdsD 634536..635522 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  DN066_RS03150 (DN066_03150) - 635536..636513 (-) 978 WP_003861852.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=298222 DN066_RS03120 WP_003861864.1 630647..632080(-) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain Pb204]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=298222 DN066_RS03120 WP_003861864.1 630647..632080(-) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain Pb204]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGACGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACTCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment