Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   ERL60_RS00390 Genome accession   NZ_CP035312
Coordinates   79032..79700 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain D72     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 74032..84700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ERL60_RS00360 (ERL60_00360) yicC 74051..74914 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  ERL60_RS00365 (ERL60_00365) rph 75041..75757 (+) 717 WP_001247089.1 ribonuclease PH -
  ERL60_RS00370 (ERL60_00370) pyrE 75823..76464 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  ERL60_RS00375 (ERL60_00375) slmA 76501..77097 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  ERL60_RS00380 (ERL60_00380) dut 77204..77662 (-) 459 WP_000976070.1 dUTP diphosphatase -
  ERL60_RS00385 (ERL60_00385) coaBC 77640..78860 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  ERL60_RS00390 (ERL60_00390) radC 79032..79700 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  ERL60_RS00395 (ERL60_00395) rpmB 79917..80153 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  ERL60_RS00400 (ERL60_00400) rpmG 80174..80341 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  ERL60_RS00405 (ERL60_00405) mutM 80439..81248 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  ERL60_RS00410 (ERL60_00410) coaD 81287..81766 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  ERL60_RS00415 (ERL60_00415) waaA 81774..83051 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  ERL60_RS00420 (ERL60_00420) rfaQ 83464..84522 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=298205 ERL60_RS00390 WP_001297375.1 79032..79700(+) (radC) [Escherichia coli strain D72]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=298205 ERL60_RS00390 WP_001297375.1 79032..79700(+) (radC) [Escherichia coli strain D72]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496