Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BCERKBAB4_RS20540 Genome accession   NC_010184
Coordinates   4099492..4100535 (-) Length   347 a.a.
NCBI ID   WP_012261710.1    Uniprot ID   -
Organism   Bacillus mycoides KBAB4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4094492..4105535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCERKBAB4_RS20495 (BcerKBAB4_4088) - 4094890..4095090 (-) 201 WP_002015009.1 YqzE family protein -
  BCERKBAB4_RS20500 (BcerKBAB4_4089) - 4095129..4095626 (-) 498 WP_012261707.1 shikimate kinase -
  BCERKBAB4_RS20505 (BcerKBAB4_4090) - 4095778..4096428 (-) 651 WP_002131210.1 2OG-Fe(II) oxygenase -
  BCERKBAB4_RS20510 (BcerKBAB4_4091) comGG 4096603..4096974 (-) 372 WP_002167676.1 competence type IV pilus minor pilin ComGG -
  BCERKBAB4_RS20515 (BcerKBAB4_4092) comGF 4096971..4097432 (-) 462 WP_002088620.1 competence type IV pilus minor pilin ComGF -
  BCERKBAB4_RS20520 (BcerKBAB4_4093) - 4097416..4097712 (-) 297 WP_002088621.1 hypothetical protein -
  BCERKBAB4_RS20525 (BcerKBAB4_4094) comGD 4097705..4098160 (-) 456 WP_002129089.1 competence type IV pilus minor pilin ComGD -
  BCERKBAB4_RS20530 (BcerKBAB4_4095) comGC 4098157..4098456 (-) 300 WP_012261708.1 competence type IV pilus major pilin ComGC -
  BCERKBAB4_RS20535 (BcerKBAB4_4096) comGB 4098468..4099505 (-) 1038 WP_012261709.1 competence type IV pilus assembly protein ComGB -
  BCERKBAB4_RS20540 (BcerKBAB4_4097) comGA 4099492..4100535 (-) 1044 WP_012261710.1 competence type IV pilus ATPase ComGA Machinery gene
  BCERKBAB4_RS20545 (BcerKBAB4_4098) - 4100741..4101436 (+) 696 WP_002014995.1 helix-turn-helix transcriptional regulator -
  BCERKBAB4_RS20550 (BcerKBAB4_4099) - 4101718..4101960 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  BCERKBAB4_RS20555 (BcerKBAB4_4100) - 4102068..4103462 (+) 1395 WP_002138338.1 L-cystine transporter -
  BCERKBAB4_RS20560 (BcerKBAB4_4101) - 4103543..4103935 (-) 393 WP_002190672.1 hypothetical protein -
  BCERKBAB4_RS20565 (BcerKBAB4_4102) - 4104023..4104238 (-) 216 WP_002067106.1 DUF3912 family protein -
  BCERKBAB4_RS20570 (BcerKBAB4_4103) - 4104491..4104802 (+) 312 WP_002129099.1 hypothetical protein -
  BCERKBAB4_RS20575 (BcerKBAB4_4104) - 4104839..4105327 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39276.79 Da        Isoelectric Point: 9.4642

>NTDB_id=29803 BCERKBAB4_RS20540 WP_012261710.1 4099492..4100535(-) (comGA) [Bacillus mycoides KBAB4]
MNSVELFANMIMKEACRVRASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLEASVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=29803 BCERKBAB4_RS20540 WP_012261710.1 4099492..4100535(-) (comGA) [Bacillus mycoides KBAB4]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCGAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATTATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTGCGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTGTGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAAGCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

42.955

83.862

0.36

Multiple sequence alignment