Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BCERKBAB4_RS19270 Genome accession   NC_010184
Coordinates   3877583..3878017 (-) Length   144 a.a.
NCBI ID   WP_002014817.1    Uniprot ID   -
Organism   Bacillus mycoides KBAB4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3872583..3883017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCERKBAB4_RS19255 (BcerKBAB4_3830) - 3873726..3874283 (+) 558 WP_002128898.1 PadR family transcriptional regulator -
  BCERKBAB4_RS19260 (BcerKBAB4_3831) metE 3874825..3877113 (+) 2289 WP_002014819.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BCERKBAB4_RS19265 (BcerKBAB4_3832) comJ 3877154..3877543 (-) 390 WP_012261629.1 competence protein ComJ -
  BCERKBAB4_RS19270 (BcerKBAB4_3833) nucA/comI 3877583..3878017 (-) 435 WP_002014817.1 DNA-entry nuclease Machinery gene
  BCERKBAB4_RS19275 (BcerKBAB4_3834) - 3878254..3878997 (+) 744 WP_002033817.1 DUF3967 domain-containing protein -
  BCERKBAB4_RS19280 (BcerKBAB4_3835) - 3879057..3880331 (-) 1275 WP_002014814.1 sensor histidine kinase -
  BCERKBAB4_RS19285 (BcerKBAB4_3836) - 3880641..3880841 (+) 201 WP_000929276.1 hypothetical protein -
  BCERKBAB4_RS19290 (BcerKBAB4_3837) - 3881076..3882239 (+) 1164 WP_002033816.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16194.59 Da        Isoelectric Point: 8.4331

>NTDB_id=29800 BCERKBAB4_RS19270 WP_002014817.1 3877583..3878017(-) (nucA/comI) [Bacillus mycoides KBAB4]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=29800 BCERKBAB4_RS19270 WP_002014817.1 3877583..3878017(-) (nucA/comI) [Bacillus mycoides KBAB4]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCAGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACGGATGCTATAAAAGAAGGACATTCGGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542

Multiple sequence alignment