Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DMR38_RS20455 Genome accession   NZ_CP029758
Coordinates   4362317..4363672 (-) Length   451 a.a.
NCBI ID   WP_127723450.1    Uniprot ID   A0A3Q9S9T8
Organism   Clostridium sp. AWRP     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4357317..4368672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DMR38_RS20435 (DMR38_20465) ispD 4358669..4359361 (-) 693 WP_127723442.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DMR38_RS20440 (DMR38_20470) - 4359365..4360492 (-) 1128 WP_127723444.1 PIN/TRAM domain-containing protein -
  DMR38_RS20445 (DMR38_20475) - 4360794..4361198 (+) 405 WP_127723446.1 DUF1573 domain-containing protein -
  DMR38_RS20450 (DMR38_20480) disA 4361238..4362302 (-) 1065 WP_127723448.1 DNA integrity scanning diadenylate cyclase DisA -
  DMR38_RS20455 (DMR38_20485) radA 4362317..4363672 (-) 1356 WP_127723450.1 DNA repair protein RadA Machinery gene
  DMR38_RS20460 (DMR38_20490) - 4363958..4366399 (-) 2442 WP_127723452.1 ATP-dependent Clp protease ATP-binding subunit -
  DMR38_RS20465 (DMR38_20495) - 4366420..4367460 (-) 1041 WP_127723454.1 protein arginine kinase -
  DMR38_RS20470 (DMR38_20500) - 4367463..4368011 (-) 549 WP_127723456.1 UvrB/UvrC motif-containing protein -
  DMR38_RS20475 (DMR38_20505) - 4368085..4368567 (-) 483 WP_127723458.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 451 a.a.        Molecular weight: 49585.50 Da        Isoelectric Point: 8.5514

>NTDB_id=295940 DMR38_RS20455 WP_127723450.1 4362317..4363672(-) (radA) [Clostridium sp. AWRP]
MVKKKTVFICQECGYESPKWLGKCPSCNKWNCMVEEIKSPQEVTFSDMKLSSTPKSINNIKSSEYERLDTGIMELNRVLG
GGIVKGSITLISGAPGIGKSTLLLQTASNIAEKYGKVLYVSGEESEEQIKMRGDRLDSLSNDLYIVSEVNIDKIKAHIEQ
TKPVFVVVDSIQTLFKESISSAPGSVSQVRETSNDIMRIGKSNNISFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
GELRILRTVKNRFGTTSEIGVFEMAGSGLKEILNPSAAFLEDREFQKEGSVIIGIMEGRRPILVEIQALVSETKAVMPRR
TAVGVDVSRLNLILAVLEKKLRITFYNCDVYVNVVGGLKIEGTFGDLGLALALISSAKSREIKLDNLLAVGEIGLTSEVR
PVSFCDRIVNEAKKMGFRNIIIPDRNKGKVNSKDINVIGISSLKEALNKVF

Nucleotide


Download         Length: 1356 bp        

>NTDB_id=295940 DMR38_RS20455 WP_127723450.1 4362317..4363672(-) (radA) [Clostridium sp. AWRP]
ATGGTAAAGAAAAAAACGGTTTTTATATGTCAGGAATGTGGATATGAATCTCCAAAGTGGCTGGGTAAATGTCCTAGTTG
TAATAAATGGAACTGCATGGTAGAAGAGATTAAGTCCCCTCAGGAAGTAACATTTTCTGATATGAAACTTAGCAGCACAC
CCAAGAGTATAAATAATATAAAATCCAGTGAATACGAAAGGCTAGATACTGGTATAATGGAATTGAATAGAGTTCTTGGA
GGAGGTATTGTAAAAGGTTCAATAACACTTATATCAGGAGCTCCTGGTATTGGAAAATCGACTTTGCTTTTACAGACGGC
CAGTAATATTGCTGAAAAGTATGGAAAAGTGCTGTATGTCTCAGGAGAAGAGTCAGAAGAACAGATAAAGATGAGGGGAG
ATAGACTTGATTCTTTGTCTAATGATCTTTATATAGTTTCAGAAGTTAATATTGATAAAATAAAGGCTCATATAGAACAA
ACTAAACCCGTATTTGTAGTAGTGGACTCTATTCAGACTTTATTTAAAGAATCTATTAGTTCAGCACCGGGTAGTGTGTC
TCAAGTAAGAGAAACTTCTAACGATATTATGCGTATAGGAAAGTCTAATAATATATCTTTTTTTATAGTGGCACATGTTA
CAAAGCAGGGTGAACTAGCAGGACCTAGGGTACTAGAACATATGGTGGATACTGTACTGTCTTTTGAAGGAGAAAGAACA
GGAGAATTAAGAATTTTAAGAACTGTAAAAAATCGTTTTGGTACTACCAGCGAAATTGGTGTATTTGAAATGGCTGGAAG
CGGGCTTAAAGAAATATTAAATCCTTCTGCAGCGTTTTTAGAAGATAGAGAATTTCAAAAAGAAGGTTCTGTAATTATTG
GTATAATGGAAGGAAGAAGACCTATACTAGTAGAAATACAAGCATTGGTTAGCGAAACTAAAGCAGTTATGCCAAGGAGA
ACAGCCGTTGGAGTAGATGTCTCTAGATTAAATTTAATTTTAGCAGTACTTGAGAAAAAACTTAGGATTACATTTTATAA
TTGTGATGTTTATGTAAATGTAGTAGGAGGACTTAAGATAGAGGGAACTTTTGGAGATTTAGGCCTTGCCCTTGCACTTA
TATCTAGTGCTAAATCTAGGGAAATAAAGCTAGATAATCTTTTGGCAGTAGGTGAAATTGGACTTACAAGTGAAGTTAGA
CCAGTATCGTTTTGTGATAGAATTGTAAATGAAGCAAAAAAAATGGGATTTAGGAATATAATAATACCAGATAGAAATAA
GGGAAAGGTAAATTCTAAAGATATAAATGTTATAGGTATATCCTCCTTGAAGGAGGCATTAAATAAAGTTTTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(59-217)

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9S9T8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.214

100

0.514
  radA Streptococcus pneumoniae Rx1

48.246

100

0.488
  radA Streptococcus pneumoniae D39

48.246

100

0.488
  radA Streptococcus pneumoniae R6

48.246

100

0.488
  radA Streptococcus pneumoniae TIGR4

48.246

100

0.488
  radA Streptococcus mitis NCTC 12261

48.026

100

0.486
  radA Streptococcus mitis SK321

48.026

100

0.486

Multiple sequence alignment