Detailed information NTG0029559

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: LHV_RS01810
• Genome accession: NC_010080
• Coordinates: 364180..365556 (+)
• Length: 458 a.a.
• NCBI ID: WP_012211406.1
• Uniprot ID: U6F1L0
• Organism: Lactobacillus helveticus DPC 4571
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	342500..365556	364180..365556	within	0

Gene organization within MGE regions

Location: 342500..365556

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LHV_RS01705 (lhv_0345)	-	342500..343729 (-)	1230	WP_012211392.1	IS110-like element ISLhe4 family transposase	-
LHV_RS01710 (lhv_0346)	-	343967..345226 (-)	1260	WP_012211393.1	ISL3 family transposase	-
LHV_RS01715 (lhv_0347)	-	345372..345782 (-)	411	WP_012211394.1	DUF805 domain-containing protein	-
LHV_RS01720 (lhv_0348)	-	345872..346456 (-)	585	WP_012211395.1	DJ-1 family glyoxalase III	-
LHV_RS01725 (lhv_0349)	-	346497..347157 (-)	661	Protein_344	HAD family hydrolase	-
LHV_RS01730 (lhv_0350)	-	347231..347989 (+)	759	WP_012211396.1	sulfite exporter TauE/SafE family protein	-
LHV_RS01735 (lhv_0352)	-	348028..348708 (+)	681	WP_003631352.1	histidine phosphatase family protein	-
LHV_RS01740 (lhv_0353)	-	348771..349670 (+)	900	WP_012211397.1	cation diffusion facilitator family transporter	-
LHV_RS11860	-	349679..349861 (+)	183	WP_014563022.1	hypothetical protein	-
LHV_RS01750 (lhv_0354)	-	349875..350555 (+)	681	WP_012211398.1	2,3-bisphosphoglycerate-dependent phosphoglycerate mutase	-
LHV_RS01755 (lhv_0355)	-	350659..351594 (+)	936	WP_003631355.1	AAA family ATPase	-
LHV_RS01760 (lhv_0356)	-	351803..352981 (+)	1179	WP_012211358.1	IS256 family transposase	-
LHV_RS01765	-	353119..354348 (+)	1230	Protein_352	RNA-guided endonuclease TnpB family protein	-
LHV_RS01770 (lhv_0360)	mgtA	354817..357495 (+)	2679	WP_023190834.1	magnesium-translocating P-type ATPase	-
LHV_RS01775 (lhv_0361)	-	357559..357964 (-)	406	Protein_354	hypothetical protein	-
LHV_RS01780 (lhv_0362)	-	358147..358989 (-)	843	WP_012211401.1	RhaT/GlcU family sugar-proton symporter	-
LHV_RS01785 (lhv_0363)	pepC	359133..360482 (-)	1350	WP_012211402.1	aminopeptidase C	-
LHV_RS01790 (lhv_0364)	-	360692..361549 (+)	858	WP_012212171.1	IS982 family transposase	-
LHV_RS01795 (lhv_0365)	-	361786..363045 (-)	1260	WP_193327843.1	RNA-guided endonuclease TnpB family protein	-
LHV_RS01800 (lhv_0366)	-	363190..363492 (-)	303	WP_003625857.1	hypothetical protein	-
LHV_RS01805 (lhv_0367)	-	363629..364180 (+)	552	WP_012211405.1	dUTP diphosphatase	-
LHV_RS01810 (lhv_0368)	radA	364180..365556 (+)	1377	WP_012211406.1	DNA repair protein RadA	Machinery gene

Sequence

Protein

• Length: 458 a.a.
• Molecular weight: 50252.70 Da
• Isoelectric Point: 6.2565

>NTDB_id=29559 LHV_RS01810 WP_012211406.1 364180..365556(+) (radA) [Lactobacillus helveticus DPC 4571]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVHLDKIKAEKEERIPTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLHIMSDLAEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHTYRILHSVKNRFGAANEIGMFEMENEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMSAASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIEARVKEAAKVGFKRIFIPKHNMYQSLTENGIEVIPVASIPQALELVFG

Nucleotide

• Length: 1377 bp

>NTDB_id=29559 LHV_RS01810 WP_012211406.1 364180..365556(+) (radA) [Lactobacillus helveticus DPC 4571]
ATGGCTAGAGTAAAAACGCAATATAAATGTCGTTCCTGTGGTTATATTTCAGCTAGTTACTTGGGTCGTTGTCCTAACTG
CGGTGCATGGAATCAATTTGAAAAAGAAACTGAAGAAGTACAGAAAAGATCTACTAAAGCAACGGCAAGCCGTTTAATTC
AAAAAACAGGTCTTAATGAACCTGTTCATTTGGATAAAATCAAGGCTGAAAAAGAAGAACGAATTCCTACTAAGTCCGAA
GAATTAAATCGTGTATTAGGTGGCGGGATCGTTCCTGGATCCCTCGTCTTAATTGGTGGGGACCCTGGTATTGGTAAGTC
AACTTTAATGTTGCATATTATGAGTGATCTGGCAGAAAAATATAAAGTGCTTTATGTTTCAGGAGAAGAGTCAGCTAATC
AAATCAAGCTGAGGGCAGATCGGCTTGGAGTTGGTCAAAGCAACATGCTTTTATATCCAGAAACAGATATGCATGATATT
CGTGAGCAGATCAATGATGTTAAGCCTGATTTTGTCGTTATTGATTCTATTCAAACGATGAATGAACCTAGTCTTGATTC
AATGACTGGTTCTGCTTCACAAGTACGTGAGGTAACAAGTGAATTGATGAAGATTGCTAAGATGGATGCAATTACTGTTT
TTGTTATTGGACACGTGACTAAAGAAGGTGCGATTGCTGGCCCCAAAATTTTGGAACACATGGTGGATACTGTCCTTTAT
TTTGAGGGGGATGAACACCACACGTACCGGATTTTGCATTCCGTAAAAAATCGTTTTGGTGCTGCTAACGAAATCGGCAT
GTTTGAAATGGAAAATGAAGGCTTACGTGAAGTAACCAATCCATCTTCGATTTTCCTTGATCAAAGATTACCTAATTCAA
CTGGGTCAGCCGTAGTAGTTTCACTTGAAGGAACGCGGCCACTTTTGGCAGAAATTCAAGCATTAGTAACGCCAACTGCA
TTTGGCTATGCCAAACGAACTACTTCAGGAATTAGTTTTAATAAAGCTTCACTATTATTAGCGGTTCTTGAAAAACGCGG
CAATTTAATGCTTCAAAATCAGGATGTTTATTTAACGGCTACTGGCGGTATTAAATTAAATGAACCTGCAATCGATCTAG
CTATTGCTATGTCAGCAGCATCAAGCTACACCGATAAAGAAATCTCACCGACTGATTGTTTTGTAGGGGAAGTGGGATTA
ACCGGTGAAGTTCGCCGCGTTGATAAAATCGAAGCTAGAGTAAAGGAAGCTGCCAAGGTAGGTTTTAAACGTATTTTTAT
TCCTAAGCACAATATGTATCAAAGTCTAACGGAAAATGGCATTGAAGTAATTCCAGTTGCCAGCATTCCACAGGCATTAG
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-34)

• Subunit ChlI of Mg-chelatase (ChlI) (363-431)

• AAA domain (AAA_25) (66-223)

3D structure

• AlphaFold DB: U6F1L0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus mitis NCTC 12261	57.112	99.782	0.57
radA	Streptococcus pneumoniae Rx1	57.112	99.782	0.57
radA	Streptococcus pneumoniae D39	57.112	99.782	0.57
radA	Streptococcus pneumoniae R6	57.112	99.782	0.57
radA	Streptococcus pneumoniae TIGR4	57.112	99.782	0.57
radA	Streptococcus mitis SK321	57.112	99.782	0.57
radA	Bacillus subtilis subsp. subtilis str. 168	55.991	100	0.561