Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DACI_RS27770 Genome accession   NC_010002
Coordinates   6092178..6093920 (+) Length   580 a.a.
NCBI ID   WP_012207286.1    Uniprot ID   -
Organism   Delftia acidovorans SPH-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6087178..6098920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DACI_RS27740 (Daci_5483) proB 6087696..6088844 (-) 1149 WP_012207281.1 glutamate 5-kinase -
  DACI_RS27745 (Daci_5484) cgtA 6088928..6090043 (-) 1116 WP_012207282.1 Obg family GTPase CgtA -
  DACI_RS27750 (Daci_5485) rpmA 6090193..6090450 (-) 258 WP_012207283.1 50S ribosomal protein L27 -
  DACI_RS27755 (Daci_5486) rplU 6090466..6090777 (-) 312 WP_012207284.1 50S ribosomal protein L21 -
  DACI_RS27760 (Daci_5487) - 6091008..6091937 (+) 930 WP_034396917.1 polyprenyl synthetase family protein -
  DACI_RS27770 (Daci_5488) pilB 6092178..6093920 (+) 1743 WP_012207286.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DACI_RS27775 (Daci_5489) pilC 6093959..6095176 (+) 1218 WP_012207287.1 type II secretion system F family protein Machinery gene
  DACI_RS27780 (Daci_5490) pilD 6095176..6096060 (+) 885 WP_012207288.1 A24 family peptidase Machinery gene
  DACI_RS27785 (Daci_5491) coaE 6096075..6096719 (+) 645 WP_012207289.1 dephospho-CoA kinase -
  DACI_RS27790 (Daci_5492) zapD 6096809..6097564 (+) 756 WP_012207290.1 cell division protein ZapD -
  DACI_RS27795 (Daci_5493) - 6097582..6097818 (+) 237 WP_085930583.1 DNA gyrase inhibitor YacG -
  DACI_RS27800 (Daci_5494) - 6097829..6098347 (-) 519 WP_012207292.1 (deoxy)nucleoside triphosphate pyrophosphohydrolase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62695.82 Da        Isoelectric Point: 5.3458

>NTDB_id=29501 DACI_RS27770 WP_012207286.1 6092178..6093920(+) (pilB) [Delftia acidovorans SPH-1]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAASKAGANRTSFIAELSNAGILSALEVAETVSTMFSAPLLDLD
AINAARLPHELLDAKIISSYRVLPLSKRGNRITVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQSKSASEA
MESYSGGGDFEFGDLKPDEVAEEKDNTAAADVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KARLIEAIGRPYGMILVTGPTGSGKTVSLYTCLNMLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALLEAGYDEADIDGSWVTYKPVGCQACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=29501 DACI_RS27770 WP_012207286.1 6092178..6093920(+) (pilB) [Delftia acidovorans SPH-1]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCTTCCGCTGTTGCATCGCTTCCCGGCATCGGGCGAGCCCTGATATCAGC
AGGCAAGGTATCGCAGCAGGCAGCGGAAGCCGCCGCCAGCAAGGCCGGCGCCAACCGCACCTCGTTCATTGCCGAGTTGA
GCAACGCGGGCATCCTCTCAGCCCTGGAAGTGGCCGAGACGGTCTCGACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCCATCAACGCCGCGCGCCTGCCGCATGAGTTGCTGGACGCCAAGATCATCAGCTCCTATCGCGTCCTCCCGTTGAGCAA
GCGCGGCAACCGCATCACGGTGGCCACAGCCGACCCGACACAACAGGAAGCTGCGGAGCAGATCAAGTTCACCACCCAGC
TAGGCGTGGATTGGATCATCGTCGAAGCCGACAAGCTGGAGCGCCTGATAGCTCAGCAGAGCAAAAGCGCCAGCGAAGCC
ATGGAGAGCTACAGCGGGGGCGGCGATTTCGAGTTCGGCGACCTCAAGCCCGACGAGGTTGCCGAGGAGAAAGACAACAC
GGCGGCAGCGGACGTCGAGGACGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACCTGCATTTCGAGCCCTACGAACATCAATATCGCGTACGCTTCCGCATTGACGGCGAGTTGCGCGAAATCGCATCA
CCGCCCATTGCCATAAAGGAGAAGTTGGCCTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAAAAACGTGTTCC
TCAAGATGGCCGCATGAAGCTCAAGGTCGGCCCGGACCGCGTCATCGATTTTCGCGTGAGCACCCTGCCCACGCTCTTTG
GCGAGAAGATCGTGATCCGTATCCTTGACCCAAGCTCGGCCAAGATGGGCATTGATGCCCTGGGCTACGAACCCGAGGAA
AAGGCACGTCTGATCGAAGCCATCGGGCGCCCCTACGGAATGATTCTGGTGACAGGCCCCACGGGCTCGGGCAAGACGGT
GTCGCTGTACACCTGCCTGAACATGCTGAACAAGGCCGGGGTGAACATCGCAACGGCAGAAGACCCTTCGGAAATCAACC
TGCCCGGCGTCAATCAGGTCAATGTGAACGAAAAGGCGGGCCTTACCTTTGCGACGGCGCTCAAGGCCTTTCTGCGCCAG
GATCCCGACATCATCATGGTCGGAGAAATCCGTGACCTAGAAACCGCCGACATCTCGATCAAGGCCGCACAGACCGGCCA
CTTGGTGCTGTCCACGCTGCACACCAACGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCCCCCTTCA
ACATCGCATCGAGCGTGATCTTGATCACCGCCCAGCGGCTGGCCAGGCGCCTGTGCCCCCAGTGCAAGGAGCCTGCGGAT
ATCCCGCGGGAGGCGCTGCTGGAGGCGGGCTATGACGAGGCGGACATCGATGGCAGCTGGGTCACCTACAAGCCCGTGGG
CTGCCAAGCATGCAACAACGGCTACAAGGGACGCGTCGGCATCTACCAGGTGATGCCGATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGCGCACTGGAGATCGCGGCACAGGCCAAGCGTGAAGGCGTACGGTCTCTGCGCGGATCCGGA
CTGCACAAGGCCAGACTAGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)

Type II/IV secretion system protein (T2SSE)

(203-470)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.536

98.103

0.545
  pilB Acinetobacter baumannii D1279779

56.028

97.241

0.545
  pilF Neisseria gonorrhoeae MS11

52.297

97.586

0.51
  pilB Legionella pneumophila strain ERS1305867

50.615

98.103

0.497
  pilB Vibrio cholerae strain A1552

46.461

95

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

45.554

95

0.433
  pilB Vibrio campbellii strain DS40M4

43.717

97.414

0.426
  pilF Thermus thermophilus HB27

38.204

97.931

0.374

Multiple sequence alignment