Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DL317_RS04585 Genome accession   NZ_CP029615
Coordinates   931624..932997 (-) Length   457 a.a.
NCBI ID   WP_035154969.1    Uniprot ID   A0A347T7Z3
Organism   Limosilactobacillus reuteri strain SKKU-OGDONS-01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 926624..937997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DL317_RS04550 (DL317_04550) secE 927099..927275 (-) 177 WP_003666294.1 preprotein translocase subunit SecE -
  DL317_RS04555 (DL317_04555) rpmG 927287..927436 (-) 150 WP_003666293.1 50S ribosomal protein L33 -
  DL317_RS04560 (DL317_04560) rlmB 927502..928251 (-) 750 WP_003667343.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DL317_RS04565 (DL317_04565) - 928241..928654 (-) 414 WP_003670544.1 Mini-ribonuclease 3 -
  DL317_RS04570 (DL317_04570) cysS 928666..930078 (-) 1413 WP_113896779.1 cysteine--tRNA ligase -
  DL317_RS04580 (DL317_04580) - 930454..931605 (-) 1152 WP_113896780.1 PIN/TRAM domain-containing protein -
  DL317_RS04585 (DL317_04585) radA 931624..932997 (-) 1374 WP_035154969.1 DNA repair protein RadA Machinery gene
  DL317_RS04590 (DL317_04590) - 933023..933559 (-) 537 WP_035154966.1 dUTP diphosphatase -
  DL317_RS04595 (DL317_04595) - 933698..933994 (+) 297 WP_162859764.1 GNAT family N-acetyltransferase -
  DL317_RS04600 (DL317_04600) rpiA 934006..934689 (+) 684 WP_113896782.1 ribose-5-phosphate isomerase RpiA -
  DL317_RS04605 (DL317_04605) - 934718..936058 (+) 1341 WP_113896783.1 aminopeptidase C -
  DL317_RS04610 (DL317_04610) - 936289..937080 (-) 792 WP_113896784.1 transporter substrate-binding domain-containing protein -
  DL317_RS04615 (DL317_04615) - 937096..937845 (-) 750 WP_113896785.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49933.20 Da        Isoelectric Point: 8.1257

>NTDB_id=294300 DL317_RS04585 WP_035154969.1 931624..932997(-) (radA) [Limosilactobacillus reuteri strain SKKU-OGDONS-01]
MAKVRTQYVCQNCGYNSPRYLGRCPNCGEWNTLVEEQVEASSAPTKKATTTLTGLVAKPQKINEIDSTETPRVKTKLNEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSDEHHRVLYVSGEESGTQIKMRAERLKVAGDDFYVYPETNMDSIR
DTIRDLKPEYVVIDSVQTMQATDVSSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLTEVKNPSEIFLEERLHDATGSAVVVSLEGTRPILVEIQALVTPTVY
GNAQRTATGLSRNRVSLIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSIASSYRDKGTQPTDAFVGEVGLT
GEIRRVNRIEQRVAEAEKLGFKRIFIPKNNLKGWKPSTNIQVIGVSTLKEALYLALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=294300 DL317_RS04585 WP_035154969.1 931624..932997(-) (radA) [Limosilactobacillus reuteri strain SKKU-OGDONS-01]
ATGGCAAAAGTTCGAACACAATATGTTTGCCAAAACTGTGGTTACAACTCACCACGCTACTTAGGCCGGTGTCCAAATTG
TGGTGAGTGGAATACTTTAGTAGAAGAACAGGTAGAAGCCAGTTCTGCACCAACAAAAAAAGCAACCACCACCTTAACCG
GCTTAGTTGCCAAACCACAGAAAATTAATGAGATTGATAGTACAGAGACTCCCCGCGTAAAAACAAAATTGAACGAGTTA
AATCGCGTTTTAGGTGGGGGAATCGTTCCAGGATCACTAATTCTAATTGGTGGGGATCCAGGAATCGGGAAATCGACCCT
CCTTTTACAAGTTTCTGGACAATTGAGCGATGAGCATCACCGGGTTTTATATGTGTCTGGAGAAGAAAGTGGGACACAGA
TTAAAATGCGGGCGGAACGGTTGAAGGTTGCTGGGGATGACTTTTATGTGTATCCAGAAACGAATATGGATAGTATTCGG
GATACTATTCGTGACCTTAAGCCAGAGTATGTCGTTATTGACTCCGTGCAAACGATGCAGGCAACGGATGTAAGTTCAGC
GATTGGTAGTGTATCGCAAATTCGTGAAGTTACCGCCCAGTTAATGCAGATTGCTAAAAGTAATAACATTACGATTTTTG
TTGTTGGTCATGTGACAAAGGGTGGTGCAATCGCCGGTCCAAAGATTTTAGAGCACATGGTTGATACGGTTTTGTATTTT
GAAGGAGATCTGCACCATACATATCGTATTTTGCGATCAGTGAAAAATCGATTTGGTTCAACCAACGAGCTTGGTATTTT
TGAGATGCATACAAATGGACTGACGGAAGTAAAAAATCCATCAGAAATCTTTTTAGAGGAACGATTACATGATGCAACTG
GTTCAGCAGTCGTTGTTTCATTAGAAGGAACGCGACCGATTTTAGTGGAAATTCAGGCGCTGGTAACACCGACCGTTTAT
GGAAATGCACAGCGAACCGCTACCGGTTTGAGTCGAAACCGAGTATCATTGATAATGGCAGTATTAGAGAAACGTGCTAA
CCTAATGCTTCAAAATCAAGATGCTTACTTAAAAGCAGCTGGGGGCGTTAAATTAGATGAGCCAGCGATTGATTTAGCAA
TTGCGGTAAGCATTGCCTCTAGTTACCGGGATAAAGGTACCCAACCAACTGACGCATTTGTCGGTGAAGTAGGTTTAACT
GGGGAAATTCGCCGGGTAAATCGCATTGAACAGCGGGTTGCTGAAGCAGAAAAACTTGGCTTTAAACGGATCTTTATTCC
CAAAAATAATTTAAAGGGTTGGAAACCATCAACTAATATTCAAGTAATAGGTGTCTCAACCCTGAAAGAAGCGTTATATT
TAGCGTTAGGGTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(65-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A347T7Z3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.593

98.906

0.659
  radA Streptococcus mitis NCTC 12261

66.593

98.906

0.659
  radA Streptococcus pneumoniae Rx1

66.372

98.906

0.656
  radA Streptococcus pneumoniae D39

66.372

98.906

0.656
  radA Streptococcus pneumoniae R6

66.372

98.906

0.656
  radA Streptococcus pneumoniae TIGR4

66.372

98.906

0.656
  radA Bacillus subtilis subsp. subtilis str. 168

63.02

100

0.63

Multiple sequence alignment