Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C0060_RS04215 Genome accession   NZ_CP029614
Coordinates   880986..882365 (-) Length   459 a.a.
NCBI ID   WP_086874642.1    Uniprot ID   -
Organism   Lactobacillus johnsonii strain Byun-jo-01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 875986..887365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0060_RS04195 (LJ1_00852) rlmB 876725..877480 (-) 756 WP_086874645.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  C0060_RS04200 (LJ1_00853) - 877467..877910 (-) 444 WP_004895772.1 ribonuclease III domain-containing protein -
  C0060_RS04205 (LJ1_00854) cysS 877903..879315 (-) 1413 WP_164084823.1 cysteine--tRNA ligase -
  C0060_RS04210 (LJ1_00855) gltX 879405..880904 (-) 1500 WP_086874643.1 glutamate--tRNA ligase -
  C0060_RS04215 (LJ1_00856) radA 880986..882365 (-) 1380 WP_086874642.1 DNA repair protein RadA Machinery gene
  C0060_RS04220 (LJ1_00857) - 882365..882916 (-) 552 WP_004895781.1 dUTP diphosphatase -
  C0060_RS04225 (LJ1_00858) - 883033..884067 (-) 1035 WP_086874641.1 LCP family protein -
  C0060_RS04230 (LJ1_00859) pepC 884264..885613 (+) 1350 WP_086874640.1 aminopeptidase C -
  C0060_RS04235 (LJ1_00860) - 885899..886912 (+) 1014 WP_044496651.1 sugar transferase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50145.73 Da        Isoelectric Point: 7.6370

>NTDB_id=294279 C0060_RS04215 WP_086874642.1 880986..882365(-) (radA) [Lactobacillus johnsonii strain Byun-jo-01]
MAKIKTRYKCRNCGYISASYLGRCPNCGAWNQFEEEIQEIRKVSTKATASRLMTKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEHSVLYVSGEESASQIKMRADRLGVSNSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAVFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVNQIEARVREAAKVGFKRIFIPKNNLSQELKNNSDIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=294279 C0060_RS04215 WP_086874642.1 880986..882365(-) (radA) [Lactobacillus johnsonii strain Byun-jo-01]
ATGGCAAAAATTAAAACTCGTTATAAGTGTCGTAATTGCGGGTATATTTCTGCTTCCTATCTTGGAAGATGTCCAAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGATACAGGAAATTAGAAAAGTATCAACTAAGGCAACTGCAAGCCGTCTGATGA
CTAAAATTGGAAATAATGATCCAGTAAAGTTAACTGAGGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGAATTGTTCCGGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACTTTAATGCTTCAAATTACTGGGGCTTTAGCTAAAGAACATAGCGTCTTATATGTTTCAGGAGAAGAGTCTGCGAGTC
AAATAAAGATGCGAGCAGATCGATTAGGAGTAAGCAATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAGAATATT
CGTGACCAGATTGATGAAATTAAGCCAGACTTTTTGGTAATTGACTCTATTCAAACAATGAATGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAATTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGACATGTAACTAAAGAAGGAGCAATTGCTGGTCCTAAGATTATGGAGCATATGGTTGATACCGTTCTTTAC
TTTGAAGGAGATGGCCATCATTCATATCGAATTTTAAGATCAGTCAAGAATCGTTTTGGCGCAGCTAATGAAATTGGAAT
GTTTGAAATGAAAAATGAAGGGTTAGCAGAAGTAAGTAATCCTTCAGCTGTCTTTTTAGATGAACGCTTACCCAATTCGA
CTGGTTCCTCAGTTGTTGTTTCACTTGAAGGAACGCGGCCTCTTCTTGCGGATATTCAAGCTTTAGTAACTCCAACTGCA
TTTGGATATGCGAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGGCAGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAATCAAGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCGATTGATCTTG
CAATTTGCATGGCGGTTGCTTCAAGTTATAAAAATAAAGAAATTTCTTCTACTGATTGCTTTGTAGGTGAAGTTGGATTA
ACTGGAGAAATTAGAAGAGTTAATCAAATCGAAGCTAGAGTCAGAGAGGCCGCAAAAGTGGGCTTTAAGAGGATTTTTAT
TCCTAAAAATAACTTAAGCCAGGAACTTAAAAATAACTCTGATATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.262

99.782

0.601
  radA Streptococcus mitis SK321

60.262

99.782

0.601
  radA Streptococcus pneumoniae Rx1

60.262

99.782

0.601
  radA Streptococcus pneumoniae D39

60.262

99.782

0.601
  radA Streptococcus pneumoniae R6

60.262

99.782

0.601
  radA Streptococcus pneumoniae TIGR4

60.262

99.782

0.601
  radA Bacillus subtilis subsp. subtilis str. 168

55.702

99.346

0.553

Multiple sequence alignment