Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C1M51_RS09990 Genome accession   NZ_CP029606
Coordinates   2069450..2070973 (-) Length   507 a.a.
NCBI ID   WP_129449459.1    Uniprot ID   A0A4P6GVN1
Organism   Methylibium sp. Pch-M     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2064450..2075973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1M51_RS09965 (C1M51_09900) - 2065227..2065832 (+) 606 WP_011827746.1 LemA family protein -
  C1M51_RS09970 (C1M51_09905) - 2065840..2066751 (+) 912 WP_129449451.1 YgcG family protein -
  C1M51_RS09975 (C1M51_09910) - 2066751..2067260 (+) 510 WP_129449453.1 TPM domain-containing protein -
  C1M51_RS09980 (C1M51_09915) - 2067298..2068068 (+) 771 WP_165349671.1 DUF4399 domain-containing protein -
  C1M51_RS09985 (C1M51_09920) - 2068097..2069362 (-) 1266 WP_129449457.1 class I SAM-dependent RNA methyltransferase -
  C1M51_RS09990 (C1M51_09925) rpoN/rpoN1 2069450..2070973 (-) 1524 WP_129449459.1 RNA polymerase factor sigma-54 Machinery gene
  C1M51_RS09995 (C1M51_09930) lptB 2070973..2071728 (-) 756 WP_011827750.1 LPS export ABC transporter ATP-binding protein -
  C1M51_RS10000 (C1M51_09935) lptA 2071725..2072339 (-) 615 WP_242499645.1 lipopolysaccharide transport periplasmic protein LptA -
  C1M51_RS10005 (C1M51_09940) - 2072447..2073091 (-) 645 WP_129449463.1 thiol:disulfide interchange protein DsbA/DsbL -
  C1M51_RS10010 (C1M51_09945) - 2073151..2073783 (-) 633 WP_129449465.1 SPOR domain-containing protein -
  C1M51_RS10015 (C1M51_09950) argS 2073795..2075480 (-) 1686 WP_129449467.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 507 a.a.        Molecular weight: 56447.02 Da        Isoelectric Point: 4.7519

>NTDB_id=294052 C1M51_RS09990 WP_129449459.1 2069450..2070973(-) (rpoN/rpoN1) [Methylibium sp. Pch-M]
MKPSLQVRFSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEVEEDAPTPFDAPVERATVSERQADEAWEGSGS
EAAADPEPVAVDAAEFGTTEREDWENGTEREDFDGIRETPGKAGNNDSDEFDPMERSSAGVSLQDHLRDQLRGMRLSDED
RGAVMVLIESLDEDGYLADPLEEIAQRLAGDEDDIAVEELLDRLRCALKWLHNLEPLGVGARDLSECLTLQLRAGPRCEA
QMIAILICKYHLELLARRDGKKLMAATGADEELLKAAQALIVRCEPKPGRPFTKAEANIIVPDVIVQKAGRGWRVVLNPD
VMPKLRINDLYAQAIKQQRGARTESGAGLSSRLQEARWFMKNILQRFDTIQRVSQAIVERQKAFFSHGAIAMKPLVLREI
ADELGLHESTISRVTTAKYMSTPYGTFELKYFFGSSLNTEAGGNASSTAVRALIKQLVSAEDAKKPLSDSQLSSMLEEQG
IQVARRTVAKYREALKIAPANLRRTMM

Nucleotide


Download         Length: 1524 bp        

>NTDB_id=294052 C1M51_RS09990 WP_129449459.1 2069450..2070973(-) (rpoN/rpoN1) [Methylibium sp. Pch-M]
ATGAAGCCTTCCCTGCAGGTCCGTTTTTCGCAGCATCTGGCGCTCACGCCGCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTTTCCACGCTCGAGCTTCACCAGGAAGTCGAGCAGATGCTGGAACAGAACCCCTTCCTCGAAGTGGAGGAGGATGCGC
CGACGCCGTTCGACGCGCCGGTGGAGCGCGCCACGGTCTCCGAACGCCAGGCCGACGAGGCCTGGGAGGGCTCGGGGTCC
GAGGCGGCCGCCGACCCCGAGCCGGTCGCGGTGGATGCGGCCGAGTTCGGCACCACCGAGCGCGAGGACTGGGAGAACGG
TACCGAGCGCGAGGACTTCGACGGCATCCGCGAGACGCCCGGCAAGGCCGGCAACAACGACAGCGACGAGTTCGACCCCA
TGGAGCGCAGCAGCGCCGGGGTGAGCCTGCAGGACCACCTGCGCGACCAGTTGCGCGGCATGCGCCTGAGCGACGAGGAC
CGCGGCGCGGTGATGGTGCTGATCGAATCGCTCGACGAGGACGGCTACCTGGCCGACCCGCTGGAAGAGATCGCCCAGCG
CCTGGCCGGCGACGAGGACGACATCGCGGTCGAGGAGCTGCTCGACCGCCTGCGCTGTGCGCTGAAGTGGCTGCACAACC
TGGAGCCGCTGGGCGTGGGGGCGCGCGACCTGTCGGAGTGCCTGACGCTGCAGCTGCGGGCCGGACCGCGCTGCGAGGCG
CAGATGATCGCGATCCTGATCTGCAAGTACCACCTCGAGCTGCTGGCGCGGCGCGACGGCAAGAAGCTGATGGCCGCCAC
CGGCGCCGACGAGGAGCTGCTGAAGGCCGCGCAGGCACTGATCGTGCGCTGCGAGCCCAAGCCCGGCCGGCCCTTCACCA
AGGCCGAGGCCAACATCATCGTGCCCGATGTCATCGTGCAGAAGGCCGGCCGCGGCTGGCGCGTCGTGCTCAACCCCGAC
GTGATGCCCAAGCTCCGCATCAACGACCTCTACGCACAGGCCATCAAGCAGCAGCGCGGCGCGCGCACCGAATCGGGCGC
GGGCCTGAGCTCGCGGCTGCAGGAGGCGCGCTGGTTCATGAAGAACATCCTGCAGCGCTTCGACACCATCCAGCGCGTGT
CGCAGGCCATCGTCGAGCGGCAGAAGGCCTTCTTCAGCCATGGCGCGATCGCGATGAAGCCGCTGGTGCTGCGCGAGATC
GCCGACGAACTGGGCCTGCACGAGTCGACCATCTCGCGCGTGACCACCGCCAAGTACATGTCCACGCCCTACGGCACCTT
CGAGCTGAAGTACTTCTTCGGCTCCTCGCTCAACACCGAGGCCGGCGGCAACGCATCGAGCACCGCGGTGCGCGCGCTGA
TCAAGCAGCTGGTCAGCGCGGAGGACGCCAAGAAGCCGCTGTCGGACAGCCAGCTCAGCAGCATGCTGGAAGAGCAGGGC
ATCCAGGTGGCGCGCCGCACGGTGGCGAAGTACCGCGAGGCGCTGAAGATCGCGCCGGCCAACCTGCGGCGCACGATGAT
GTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-331)

Sigma-54, DNA binding domain (Sigma54_DBD)

(350-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P6GVN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.817

100

0.556

Multiple sequence alignment