Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C1M51_RS07680 Genome accession   NZ_CP029606
Coordinates   1602936..1604300 (-) Length   454 a.a.
NCBI ID   WP_129448924.1    Uniprot ID   A0A4P6H010
Organism   Methylibium sp. Pch-M     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1597936..1609300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1M51_RS07640 (C1M51_07590) - 1598126..1598818 (+) 693 WP_129448918.1 anti-sigma factor domain-containing protein -
  C1M51_RS07645 (C1M51_07595) - 1598922..1599467 (+) 546 WP_036236145.1 DUF3455 domain-containing protein -
  C1M51_RS07650 (C1M51_07600) waaF 1599493..1600521 (-) 1029 WP_129448919.1 lipopolysaccharide heptosyltransferase II -
  C1M51_RS07655 (C1M51_07605) - 1600538..1600741 (-) 204 WP_129448920.1 zinc-finger domain-containing protein -
  C1M51_RS07660 (C1M51_07610) - 1600770..1601693 (-) 924 WP_011831125.1 branched-chain amino acid transaminase -
  C1M51_RS07665 (C1M51_07615) - 1601903..1602160 (+) 258 WP_129448921.1 PEP-CTERM sorting domain-containing protein -
  C1M51_RS07670 (C1M51_07620) - 1602170..1602571 (-) 402 WP_129448922.1 hypothetical protein -
  C1M51_RS07675 (C1M51_07625) - 1602565..1602939 (-) 375 WP_129448923.1 antibiotic biosynthesis monooxygenase -
  C1M51_RS07680 (C1M51_07630) radA 1602936..1604300 (-) 1365 WP_129448924.1 DNA repair protein RadA Machinery gene
  C1M51_RS07685 (C1M51_07635) arfB 1604323..1604733 (-) 411 WP_129448925.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  C1M51_RS07690 (C1M51_07640) - 1604730..1605218 (-) 489 WP_129448926.1 acyl-CoA thioesterase -
  C1M51_RS07695 (C1M51_07645) - 1605218..1605715 (-) 498 WP_129448927.1 hypothetical protein -
  C1M51_RS07700 (C1M51_07650) - 1605732..1605983 (-) 252 WP_011831133.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  C1M51_RS07705 (C1M51_07655) - 1606072..1606476 (-) 405 WP_129448928.1 DUF2177 family protein -
  C1M51_RS07710 (C1M51_07660) alr 1606562..1607665 (-) 1104 WP_129448929.1 alanine racemase -
  C1M51_RS07715 (C1M51_07665) lplT 1607846..1609123 (+) 1278 WP_129448930.1 lysophospholipid transporter LplT -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 47959.18 Da        Isoelectric Point: 7.3949

>NTDB_id=294046 C1M51_RS07680 WP_129448924.1 1602936..1604300(-) (radA) [Methylibium sp. Pch-M]
MAKDKTNYTCSECGGTSPKWLGKCPACGAWNTLEETVAEPAAATRHRFQSLARSQPVATLSEIDAADVARTPTSIEELDR
VLGGGIVAGGVVLIGGDPGIGKSTLLLQAVDALSAQMKVLYVSGEESGAQLALRSRRLGLDGSRVRVLAEIHLEKIQATI
DAEQPAFCVIDSIQTVYSDQLTSAPGSVAQVRECAAQLTRTAKSSGTSIVLVGHVTKEGALAGPRVLEHIVDTVLYFEGD
THSSFRLVRAIKNRYGAVNEIGVFAMTERGLKGVANPSAIFLSTHGEPVPGSCVMVTLEGTRPLLVEIQALVDSGGPSPR
RLSVGLERDRLAMLLAVLHRHAGIACMDQDVFVNAVGGVRISEPAADLAVMLAIQSSLRGRPLPRGFIAFGEVGLAGEVR
PAPRGQERLKEAAKLGFSVAVLPKANAPKKPIEGLRIHAVERVEQAVDVLRELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=294046 C1M51_RS07680 WP_129448924.1 1602936..1604300(-) (radA) [Methylibium sp. Pch-M]
ATGGCCAAGGACAAGACGAACTACACCTGCAGCGAATGCGGCGGCACGAGCCCCAAGTGGCTCGGCAAGTGTCCGGCCTG
CGGGGCCTGGAACACGCTGGAGGAGACCGTCGCCGAGCCGGCCGCGGCCACGCGGCACCGCTTCCAGTCGCTGGCACGCA
GCCAGCCGGTGGCCACGCTCAGCGAGATCGACGCGGCCGATGTCGCGCGCACGCCCACCAGCATCGAGGAACTCGACCGT
GTTCTCGGCGGCGGCATCGTGGCCGGCGGCGTGGTGCTGATCGGCGGCGACCCGGGCATTGGCAAGTCGACGCTGCTACT
GCAGGCGGTCGATGCGCTGTCGGCGCAGATGAAGGTGCTGTACGTCAGCGGCGAGGAGAGCGGCGCGCAGCTCGCGCTGC
GCTCGCGCCGGCTCGGCCTGGACGGCAGCCGCGTGCGGGTGCTGGCCGAGATCCATCTCGAGAAGATCCAGGCCACGATC
GACGCCGAGCAGCCGGCCTTCTGCGTGATCGACTCGATCCAGACCGTCTATTCGGACCAGCTCACGTCGGCCCCGGGCTC
GGTGGCCCAGGTGCGCGAGTGCGCCGCCCAGCTCACGCGCACCGCCAAATCCAGCGGCACCAGCATCGTGCTGGTCGGCC
ACGTGACGAAGGAGGGCGCGCTGGCCGGGCCGCGCGTGCTGGAGCACATCGTCGACACCGTGCTGTACTTCGAGGGCGAC
ACCCACAGCAGCTTCCGTCTCGTGCGGGCCATCAAGAACCGCTACGGCGCGGTCAACGAGATCGGCGTGTTCGCGATGAC
CGAGCGCGGTCTCAAGGGCGTGGCCAATCCGAGCGCGATCTTCCTCTCGACCCACGGCGAACCGGTCCCGGGCTCCTGCG
TGATGGTCACGCTCGAAGGCACCCGCCCGCTGCTGGTGGAGATCCAGGCGCTGGTCGACAGCGGCGGGCCCAGTCCGCGG
CGCCTGTCGGTCGGCCTGGAGCGCGACCGACTCGCGATGCTGCTGGCAGTGCTGCACCGCCACGCCGGCATCGCCTGCAT
GGACCAGGACGTGTTCGTCAACGCGGTCGGCGGCGTGCGCATCAGCGAGCCGGCGGCCGACCTGGCGGTGATGCTGGCGA
TCCAGAGCTCGCTGCGGGGGCGGCCGCTGCCGCGCGGCTTCATCGCCTTCGGCGAGGTCGGTCTGGCCGGCGAGGTGCGA
CCGGCGCCGCGCGGCCAGGAGCGCCTGAAAGAGGCGGCCAAGCTGGGGTTCAGTGTCGCGGTGCTGCCGAAGGCCAATGC
ACCGAAGAAGCCGATCGAGGGCCTGCGGATCCACGCCGTCGAGCGCGTCGAGCAGGCGGTCGACGTGCTGCGGGAGCTTT
CATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P6H010

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.904

100

0.491
  radA Streptococcus mitis NCTC 12261

45.796

99.559

0.456
  radA Streptococcus pneumoniae Rx1

47.765

93.612

0.447
  radA Streptococcus pneumoniae D39

47.765

93.612

0.447
  radA Streptococcus pneumoniae R6

47.765

93.612

0.447
  radA Streptococcus pneumoniae TIGR4

47.765

93.612

0.447
  radA Streptococcus mitis SK321

47.765

93.612

0.447

Multiple sequence alignment