Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CLOS_RS02380 Genome accession   NC_009922
Coordinates   522707..524077 (+) Length   456 a.a.
NCBI ID   WP_012158334.1    Uniprot ID   A8MLV2
Organism   Alkaliphilus oremlandii OhILAs     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 517707..529077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLOS_RS02365 (Clos_0454) - 518566..519072 (+) 507 WP_012158331.1 UvrB/UvrC motif-containing protein -
  CLOS_RS02370 (Clos_0455) - 519083..520105 (+) 1023 WP_012158332.1 protein arginine kinase -
  CLOS_RS02375 (Clos_0456) - 520136..522577 (+) 2442 WP_012158333.1 ATP-dependent Clp protease ATP-binding subunit -
  CLOS_RS02380 (Clos_0457) radA 522707..524077 (+) 1371 WP_012158334.1 DNA repair protein RadA Machinery gene
  CLOS_RS02385 (Clos_0458) disA 524187..525263 (+) 1077 WP_012158335.1 DNA integrity scanning diadenylate cyclase DisA -
  CLOS_RS02390 (Clos_0459) - 525631..526026 (-) 396 WP_012158336.1 hypothetical protein -
  CLOS_RS02395 (Clos_0460) - 526414..526917 (+) 504 WP_012158337.1 CarD family transcriptional regulator -
  CLOS_RS02400 (Clos_0461) - 527079..528185 (+) 1107 WP_012158338.1 PIN/TRAM domain-containing protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50368.41 Da        Isoelectric Point: 6.5131

>NTDB_id=29378 CLOS_RS02380 WP_012158334.1 522707..524077(+) (radA) [Alkaliphilus oremlandii OhILAs]
MAKIKSKFVCQECGYESVKWLGKCPGCNNWNTMEEEMLESKKDFKRSPTIVSQSKPQPIKEVKSGAYERYDTQINELNRV
LGGGLVKGSLTLITGEPGIGKSTLILQASSTIANRYGKTLYVSGEESEEQIKMRGERLNSLSDDLYIVSETNVDVIEKYI
EEYAPVFIIIDSIQTLFKEDLSSAPGSVSQVKECANNLMRIGKSKNIPMFIVAHVTKQGELAGPRVLEHMVDTVLHFEGE
RTQEFRILRALKNRFGTTSEIGVFEMREEGLVEVSNPSAMFLESLSTEAEGAIVVVTVEGTRPLLVEIQALVAPTNAGFP
RRAAVGIDINRLNLIIAVLEKKIGLPLMNQDIYVNVVGGLKLEGTSADLGVAMAIYSSMRGIPIASRAMVVMGEISLTGE
LRPISHLEKMLKEAEKMGFTRSVIPQKNKFKFDGIHMKCKGVDTLKDALEMLTLHN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=29378 CLOS_RS02380 WP_012158334.1 522707..524077(+) (radA) [Alkaliphilus oremlandii OhILAs]
ATGGCAAAGATTAAAAGTAAATTTGTGTGTCAAGAATGTGGGTATGAAAGTGTAAAGTGGCTTGGGAAATGTCCGGGATG
CAATAATTGGAATACCATGGAGGAGGAGATGCTGGAGAGCAAAAAGGATTTTAAGCGTTCACCAACCATTGTCAGCCAAT
CGAAGCCCCAGCCAATTAAGGAAGTAAAGTCTGGGGCCTATGAGCGATATGACACTCAAATTAATGAACTCAATAGGGTT
TTAGGCGGCGGTTTAGTCAAGGGATCCTTGACTTTAATCACAGGGGAACCGGGAATCGGAAAATCTACCCTGATATTACA
GGCCAGTAGCACCATTGCAAACCGGTATGGAAAAACGCTATATGTATCGGGGGAGGAATCAGAAGAGCAGATTAAAATGA
GAGGAGAACGATTGAACTCACTTTCTGACGATTTGTACATCGTTTCTGAAACCAATGTGGATGTGATTGAGAAGTATATT
GAAGAGTATGCGCCCGTATTCATCATCATCGATTCCATTCAAACTTTGTTTAAGGAGGATCTTTCTTCGGCGCCGGGCAG
TGTATCCCAAGTGAAAGAATGTGCTAATAATTTAATGAGAATTGGTAAATCTAAGAATATTCCCATGTTCATTGTAGCCC
ATGTAACGAAACAGGGAGAACTAGCAGGTCCAAGGGTTTTAGAGCATATGGTTGACACTGTATTGCATTTTGAAGGTGAA
AGAACTCAAGAGTTTCGGATACTGAGGGCTTTAAAAAATAGATTTGGAACCACCAGCGAAATTGGTGTTTTTGAAATGAG
AGAAGAAGGATTGGTTGAAGTCAGCAACCCATCCGCCATGTTCTTAGAATCTTTAAGCACGGAGGCGGAAGGGGCCATTG
TGGTTGTGACCGTAGAGGGAACGAGACCTCTTTTGGTGGAAATACAAGCTTTAGTTGCACCTACCAATGCAGGGTTTCCA
CGGCGCGCTGCTGTAGGGATTGATATCAATCGATTGAATTTGATTATTGCAGTTTTAGAGAAGAAAATAGGACTGCCTTT
GATGAATCAAGATATTTATGTCAATGTGGTAGGGGGATTAAAGCTAGAAGGCACCTCGGCAGATTTGGGTGTTGCTATGG
CCATCTACTCCAGTATGAGAGGAATTCCAATCGCTTCTAGAGCAATGGTGGTCATGGGAGAGATCAGCTTAACTGGTGAG
CTAAGACCCATCAGCCATTTGGAGAAAATGCTAAAGGAAGCAGAAAAAATGGGCTTTACCCGTTCTGTGATACCACAGAA
AAATAAGTTTAAGTTCGATGGGATTCATATGAAATGTAAAGGTGTGGATACTTTGAAGGATGCTTTGGAGATGCTAACAT
TGCATAATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(342-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8MLV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

54.018

98.246

0.531
  radA Streptococcus pneumoniae Rx1

50.22

99.561

0.5
  radA Streptococcus pneumoniae D39

50.22

99.561

0.5
  radA Streptococcus pneumoniae R6

50.22

99.561

0.5
  radA Streptococcus pneumoniae TIGR4

50.22

99.561

0.5
  radA Streptococcus mitis NCTC 12261

50.22

99.561

0.5
  radA Streptococcus mitis SK321

50.22

99.561

0.5

Multiple sequence alignment