Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DK843_RS04610 Genome accession   NZ_CP029554
Coordinates   996607..997968 (-) Length   453 a.a.
NCBI ID   WP_114063386.1    Uniprot ID   A0A344UEH0
Organism   Chromobacterium phragmitis strain IIBBL 274-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 991607..1002968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK843_RS04585 (DK843_04585) - 993000..993974 (+) 975 WP_114072665.1 hypothetical protein -
  DK843_RS04590 (DK843_04590) - 993981..994775 (-) 795 WP_114072666.1 transporter substrate-binding domain-containing protein -
  DK843_RS04595 (DK843_04595) - 995035..995388 (+) 354 WP_114063383.1 DUF2325 domain-containing protein -
  DK843_RS22975 - 995420..995782 (-) 363 WP_145964293.1 hypothetical protein -
  DK843_RS24175 (DK843_04600) - 995848..996045 (-) 198 WP_114063384.1 zf-HC2 domain-containing protein -
  DK843_RS04605 (DK843_04605) - 996042..996602 (-) 561 WP_114063385.1 sigma-70 family RNA polymerase sigma factor -
  DK843_RS04610 (DK843_04610) radA 996607..997968 (-) 1362 WP_114063386.1 DNA repair protein RadA Machinery gene
  DK843_RS04615 (DK843_04615) - 998042..998575 (-) 534 WP_114063387.1 hypothetical protein -
  DK843_RS04620 (DK843_04620) - 998590..999351 (-) 762 WP_114063388.1 SDR family oxidoreductase -
  DK843_RS04625 (DK843_04625) hpnE 999348..1000619 (-) 1272 WP_168191755.1 hydroxysqualene dehydroxylase HpnE -
  DK843_RS04630 (DK843_04630) hpnD 1000616..1001461 (-) 846 WP_114063390.1 presqualene diphosphate synthase HpnD -
  DK843_RS04635 (DK843_04635) hpnC 1001526..1002338 (-) 813 WP_114063391.1 squalene synthase HpnC -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48724.00 Da        Isoelectric Point: 6.8122

>NTDB_id=293454 DK843_RS04610 WP_114063386.1 996607..997968(-) (radA) [Chromobacterium phragmitis strain IIBBL 274-1]
MAKNKTVFSCTECGGQSPKWQGQCPHCNAWNTLVEAVAAPAAAGPRFQSWAANVTKVQKLSEVQTEETPRDPSGIDELDR
VLGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGQNRKVLYVSGEESAQQIALRASRLALDTSNVDLLAEICIENILATL
KREQPDVVVIDSIQTLYTEQVTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
SHSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEGTRPLLVEIQALVDDCHGFQP
KRLTVGLEQNRLAMLLAVLHRHGGVACFDQDVFLNAVGGVKINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEV
RPVTRGQERLKEAAKLGFTRAIVPSANKPRQEIEGLKVLAVDRLDQAVEYCRE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=293454 DK843_RS04610 WP_114063386.1 996607..997968(-) (radA) [Chromobacterium phragmitis strain IIBBL 274-1]
ATGGCCAAGAACAAAACCGTATTCAGCTGCACCGAGTGCGGCGGACAATCGCCCAAATGGCAGGGCCAATGCCCGCATTG
CAACGCCTGGAACACGCTGGTGGAGGCCGTGGCCGCGCCGGCGGCGGCCGGCCCGCGCTTCCAGTCCTGGGCGGCTAACG
TCACCAAGGTGCAGAAGCTGTCCGAGGTGCAGACCGAGGAAACCCCGCGCGATCCGTCCGGCATCGACGAGCTGGACCGC
GTGCTGGGCGGCGGCATCGTCCGCGGCGCGGTGGTGCTGATCGGCGGCGACCCCGGCATCGGCAAGTCCACGCTGCTGTT
GCAGGCGCTGTCGCAGATCGGGCAAAACCGCAAGGTATTGTATGTGTCCGGCGAGGAATCGGCGCAGCAGATCGCGCTGC
GCGCCTCGAGGCTGGCGCTGGATACCAGCAACGTCGACCTGCTGGCCGAGATCTGCATCGAGAACATCCTGGCCACGCTG
AAACGCGAGCAGCCGGACGTGGTGGTGATCGACTCGATCCAGACCCTGTACACCGAGCAGGTGACTTCGGCGCCGGGCTC
GGTGTCGCAGGTGCGCGAGTGCGCGGCGCAACTGACGCGGATGGCCAAGCAGAGCGGCATCACCGTGCTGCTGGTCGGCC
ACGTCACCAAGGAAGGCTCGCTGGCCGGCCCGCGCGTGCTGGAGCACATGGTGGACACCGTGCTGTATTTCGAGGGCGAC
TCCCATTCCAGCTACCGGATGATACGCGCGATCAAGAACCGCTTCGGCGCGGTCAACGAGCTGGGCGTGTTCGCGATGAC
CGATCGCGGCCTGAAGGGCGTGTCCAACCCGTCGGCCATCTTCCTCTCATCGTACCGGGATGACGTGGCCGGCTCCTGCG
TGCTGGTGACGCAGGAAGGCACGCGGCCGCTGCTGGTGGAGATCCAGGCGCTGGTGGATGACTGCCACGGCTTCCAGCCC
AAGCGCCTTACCGTCGGCCTGGAGCAGAACCGGCTGGCGATGCTGCTGGCGGTGCTGCATCGCCACGGCGGCGTGGCCTG
CTTCGACCAGGACGTGTTCCTCAACGCGGTGGGCGGCGTCAAGATCAACGAACCGGCGGCCGACCTGGCCATCATCCTGG
CCATGGTTTCGTCCTTGCGCAACAAGGCGCTGCCGGAGAAACTGGTGGTGTTCGGCGAGGTGGGCCTGGCTGGCGAGGTG
CGGCCGGTGACGCGCGGGCAGGAACGGTTGAAGGAAGCCGCCAAACTCGGCTTCACCCGCGCCATCGTGCCTTCCGCCAA
CAAGCCGCGGCAGGAAATAGAAGGCCTGAAGGTATTGGCCGTGGACCGCCTGGACCAGGCGGTGGAATATTGCAGGGAGT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(358-446)

AAA domain (AAA_25)

(67-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A344UEH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50

100

0.503
  radA Streptococcus mitis SK321

48.451

99.779

0.483
  radA Streptococcus mitis NCTC 12261

48.23

99.779

0.481
  radA Streptococcus pneumoniae TIGR4

50.472

93.598

0.472
  radA Streptococcus pneumoniae D39

50.472

93.598

0.472
  radA Streptococcus pneumoniae Rx1

50.472

93.598

0.472
  radA Streptococcus pneumoniae R6

50.472

93.598

0.472

Multiple sequence alignment