Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DKK66_RS03545 Genome accession   NZ_CP029539
Coordinates   765581..767005 (+) Length   474 a.a.
NCBI ID   WP_131355576.1    Uniprot ID   A0A4P6TGD2
Organism   Aquitalea sp. USM4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 760581..772005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKK66_RS03520 (DKK66_03510) - 762228..763208 (+) 981 WP_131355573.1 KpsF/GutQ family sugar-phosphate isomerase -
  DKK66_RS03525 (DKK66_03515) - 763227..763751 (+) 525 WP_045845551.1 HAD family hydrolase -
  DKK66_RS03530 (DKK66_03520) lptC 763748..764308 (+) 561 WP_045845550.1 LPS export ABC transporter periplasmic protein LptC -
  DKK66_RS03535 (DKK66_03525) lptA 764298..764849 (+) 552 WP_045845549.1 lipopolysaccharide transport periplasmic protein LptA -
  DKK66_RS03540 (DKK66_03530) lptB 764846..765577 (+) 732 WP_045845548.1 LPS export ABC transporter ATP-binding protein -
  DKK66_RS03545 (DKK66_03535) rpoN/rpoN1 765581..767005 (+) 1425 WP_131355576.1 RNA polymerase factor sigma-54 Machinery gene
  DKK66_RS03550 (DKK66_03540) hpf 767077..767403 (+) 327 WP_045845546.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DKK66_RS03555 (DKK66_03545) ptsN 767525..767989 (+) 465 WP_045845545.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DKK66_RS03560 (DKK66_03550) hprK 767982..768938 (+) 957 WP_045845544.1 HPr(Ser) kinase/phosphatase -
  DKK66_RS03565 (DKK66_03555) rapZ 768919..769761 (+) 843 WP_045845543.1 RNase adapter RapZ -
  DKK66_RS03570 (DKK66_03560) - 769827..770447 (+) 621 WP_045845542.1 flavin prenyltransferase UbiX -
  DKK66_RS03575 (DKK66_03565) - 770450..771910 (+) 1461 WP_131355579.1 NAD(P)H-hydrate dehydratase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 52523.32 Da        Isoelectric Point: 4.6750

>NTDB_id=293344 DKK66_RS03545 WP_131355576.1 765581..767005(+) (rpoN/rpoN1) [Aquitalea sp. USM4]
MKQSLQLRVSQQLTLTPQLQQSIKLLQLSTLDLQAEVERFLLDNPMLERGDEPPSHDSSPAETPAAQEESTSTASDSSDG
DNSQDASNGELTDWGSGSRRNDGDDEFDPMLNVPCPVSLREHLLAQLGELTLPTRDQAIVQLLIEELDDNGFLTTDLEEL
ASNLPLELELETDELMVGLRLLQQFDPPGIAARNLAESLGLQLARLPANEPGHALARQIVAEHLTLLGNRDYTRLKRILG
VDDEAIREAQQLIARLTPHPASGFGGEDVHYVIPDVTVRKRRGHWVAELNNGAVPRLRVNQLYAQMLSENRAGAGELTGR
LQEAKWLVKNIQQRFDTILKVSEAIVERQQAFFEHGEVAMRPLILRDIADELGLHESTVSRVTTQKYLLCPRGLFELKYF
FGSALETDSGGECSATAIKAHIRRMIDGENPAKPLSDSAIADELGKQGIQVARRTVAKYREAMQIPPVNLRKAL

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=293344 DKK66_RS03545 WP_131355576.1 765581..767005(+) (rpoN/rpoN1) [Aquitalea sp. USM4]
ATGAAACAGTCACTACAGCTGCGGGTCTCCCAGCAACTCACCCTCACGCCGCAACTGCAACAGTCCATCAAACTGTTGCA
GCTGTCCACGCTCGACCTGCAGGCAGAAGTCGAACGCTTCTTGCTGGACAACCCCATGCTGGAACGCGGCGATGAGCCGC
CCTCGCATGACAGCAGCCCGGCAGAAACGCCGGCTGCCCAGGAAGAATCCACCAGCACGGCGTCGGACAGCAGCGATGGT
GACAACAGCCAGGACGCCAGCAATGGCGAACTCACCGACTGGGGCAGTGGCAGCCGCCGCAACGACGGGGACGATGAATT
CGACCCCATGCTCAACGTACCCTGCCCGGTCAGCCTGCGCGAACACCTGCTGGCGCAGCTGGGTGAACTGACCCTGCCCA
CCCGCGATCAGGCCATTGTCCAGCTGTTGATCGAAGAACTGGACGACAACGGTTTCCTGACTACCGATCTGGAAGAACTG
GCCAGTAATCTGCCGCTGGAACTGGAACTGGAAACCGATGAACTGATGGTCGGGCTGCGCCTGTTACAGCAATTCGACCC
TCCCGGCATTGCCGCGCGCAACCTGGCCGAGTCGCTTGGCCTGCAACTGGCCCGTTTGCCCGCCAACGAGCCCGGCCATG
CACTGGCACGCCAGATTGTGGCCGAGCACCTGACGCTGCTGGGCAACCGCGACTACACCCGCCTGAAGCGCATTCTGGGC
GTGGATGACGAAGCCATCCGCGAGGCACAGCAACTGATCGCCCGGCTGACCCCTCACCCGGCGTCCGGTTTCGGCGGCGA
AGACGTGCACTATGTCATCCCGGATGTCACCGTGCGCAAACGGCGTGGTCACTGGGTGGCTGAACTCAATAACGGTGCGG
TACCGCGCTTGCGCGTCAACCAGCTGTATGCCCAGATGCTGTCGGAAAACCGCGCTGGCGCAGGCGAACTGACTGGCCGG
CTGCAAGAAGCCAAATGGCTGGTCAAGAACATCCAGCAACGATTTGACACCATTCTGAAAGTGTCAGAAGCTATAGTCGA
AAGACAGCAAGCGTTCTTTGAACACGGTGAAGTCGCCATGCGGCCGCTTATCCTGCGGGACATCGCAGACGAACTTGGCC
TGCATGAATCGACCGTATCACGCGTCACCACGCAGAAATACCTGCTTTGCCCTCGTGGTCTGTTCGAGCTGAAGTATTTC
TTTGGCAGTGCACTGGAAACCGACAGCGGCGGCGAATGCTCGGCAACGGCCATCAAGGCGCATATCCGCCGCATGATAGA
CGGTGAAAACCCGGCCAAGCCACTTTCGGACAGCGCCATTGCCGACGAACTGGGAAAGCAAGGGATTCAGGTTGCAAGAC
GCACCGTTGCGAAGTATCGTGAAGCTATGCAGATACCGCCGGTCAATCTGCGCAAGGCATTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P6TGD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.39

100

0.555

Multiple sequence alignment