Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DKK66_RS01285 Genome accession   NZ_CP029539
Coordinates   284712..286076 (-) Length   454 a.a.
NCBI ID   WP_131354823.1    Uniprot ID   -
Organism   Aquitalea sp. USM4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 279712..291076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKK66_RS01250 (DKK66_01250) - 279811..280023 (-) 213 WP_045847223.1 hypothetical protein -
  DKK66_RS01255 (DKK66_01255) - 280099..280884 (-) 786 WP_131354811.1 C40 family peptidase -
  DKK66_RS01260 (DKK66_01260) - 280945..282627 (+) 1683 WP_045847174.1 AarF/ABC1/UbiB kinase family protein -
  DKK66_RS01265 (DKK66_01265) - 282628..283407 (+) 780 WP_131354814.1 hypothetical protein -
  DKK66_RS01270 (DKK66_01270) - 283533..283886 (+) 354 WP_131354817.1 DUF2325 domain-containing protein -
  DKK66_RS01275 (DKK66_01275) - 283973..284152 (-) 180 WP_045847171.1 zf-HC2 domain-containing protein -
  DKK66_RS01280 (DKK66_01280) - 284149..284709 (-) 561 WP_131354820.1 sigma-70 family RNA polymerase sigma factor -
  DKK66_RS01285 (DKK66_01285) radA 284712..286076 (-) 1365 WP_131354823.1 DNA repair protein RadA Machinery gene
  DKK66_RS01290 (DKK66_01290) - 286099..286698 (-) 600 WP_131354826.1 hypothetical protein -
  DKK66_RS01295 (DKK66_01295) - 286755..287513 (-) 759 WP_131354829.1 SDR family oxidoreductase -
  DKK66_RS01300 (DKK66_01300) hpnE 287534..288838 (-) 1305 WP_131354832.1 hydroxysqualene dehydroxylase HpnE -
  DKK66_RS01305 (DKK66_01305) hpnD 288846..289691 (-) 846 WP_131354835.1 presqualene diphosphate synthase HpnD -
  DKK66_RS01310 (DKK66_01310) hpnC 289757..290569 (-) 813 WP_131354838.1 squalene synthase HpnC -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48926.34 Da        Isoelectric Point: 7.1457

>NTDB_id=293341 DKK66_RS01285 WP_131354823.1 284712..286076(-) (radA) [Aquitalea sp. USM4]
MAKTKTVFSCTECGGQVPKWQGQCPHCSAWNTLVEAVSTPASSNPRFQSWSTTNARVQVLSEVKTEEVPRDPSGIDELDR
VLGGGIVRGAVILIGGDPGIGKSTLLLQALAVIGKRRKVLYVSGEESPQQIALRASRLAVDPSQVNLLAEIRLEAILETL
KQEQPEVAVIDSIQTLYTEQVTSAPGSVSQVRECAAQLTRMAKQTGTTILLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
THSSHRMIRAIKNRFGAVNELGVFVMTETGLKGVSNPSAIFLSSYRDDVPGSCVLVTQEGTRPLLVEVQALVDDCHGMQP
KRLTVGLEQNRLAMLLAVLHRHGGVACFDQDVFLNAVGGVKISEPAADLAVILAMVSSLRNKPLPEKLVVFGEVGLAGEV
RPVSRGQERLKEAAKLGFTRALVPTANKPRQPIEGLQVITVDRLEHAVDYCRGD

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=293341 DKK66_RS01285 WP_131354823.1 284712..286076(-) (radA) [Aquitalea sp. USM4]
ATGGCAAAAACCAAAACAGTTTTCAGCTGCACCGAGTGCGGCGGCCAAGTGCCGAAATGGCAGGGCCAGTGTCCGCATTG
TTCCGCCTGGAACACCCTGGTAGAGGCCGTCTCCACGCCCGCCAGCAGCAATCCGCGTTTCCAGTCGTGGTCCACCACCA
ATGCCCGTGTGCAAGTGCTGTCCGAGGTCAAGACCGAAGAAGTGCCGCGTGACCCGTCCGGCATCGACGAACTGGACCGC
GTGCTGGGTGGCGGCATCGTGCGTGGTGCCGTCATCCTGATCGGCGGCGACCCCGGCATTGGCAAATCCACCCTGCTGTT
GCAGGCGCTGGCGGTAATCGGCAAGCGGCGCAAGGTGCTGTATGTGTCGGGTGAGGAATCGCCGCAACAGATTGCCCTGC
GGGCCTCACGGCTGGCGGTTGACCCCAGCCAGGTGAACCTGCTGGCGGAAATCCGCCTGGAAGCCATTCTGGAAACGCTC
AAGCAAGAGCAGCCGGAAGTCGCCGTCATCGACTCCATCCAGACCTTGTACACCGAGCAGGTAACTTCGGCGCCCGGTTC
GGTATCGCAGGTGCGTGAATGTGCGGCCCAGCTCACCCGCATGGCCAAGCAGACCGGCACCACCATCCTGCTGGTAGGCC
ATGTCACCAAGGAAGGCTCGCTGGCCGGCCCGCGCGTGCTGGAACACATGGTGGACACCGTGCTGTATTTCGAAGGCGAT
ACCCACTCCAGTCACCGCATGATCCGCGCCATCAAGAACCGCTTTGGCGCGGTCAACGAGCTGGGTGTGTTCGTGATGAC
CGAAACCGGGCTCAAGGGCGTGTCAAACCCCTCGGCCATTTTCCTGTCGTCCTACCGCGACGATGTTCCCGGTTCTTGCG
TGCTGGTGACGCAGGAAGGCACGCGCCCCTTGCTGGTGGAAGTGCAGGCGCTGGTGGATGACTGTCACGGCATGCAACCC
AAGCGCCTCACCGTGGGGCTGGAGCAGAACCGGCTGGCCATGTTGCTGGCGGTGCTGCACCGCCATGGCGGCGTGGCCTG
TTTCGATCAGGATGTATTCCTGAACGCCGTGGGCGGGGTCAAGATCAGCGAACCGGCGGCCGATCTGGCCGTCATCCTGG
CCATGGTGTCGTCGCTGCGTAACAAGCCCCTGCCGGAAAAACTGGTGGTATTTGGTGAAGTGGGGCTGGCCGGCGAAGTG
CGTCCGGTATCGCGCGGCCAGGAGCGCCTGAAAGAGGCGGCCAAACTGGGCTTCACCCGCGCACTGGTGCCCACGGCCAA
CAAGCCGCGTCAGCCGATTGAAGGTTTGCAAGTCATTACCGTCGACCGGCTGGAACATGCGGTTGATTACTGCCGTGGAG
ACTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.024

100

0.498
  radA Streptococcus mitis SK321

47.788

99.559

0.476
  radA Streptococcus mitis NCTC 12261

47.545

98.678

0.469
  radA Streptococcus pneumoniae TIGR4

49.057

93.392

0.458
  radA Streptococcus pneumoniae D39

49.057

93.392

0.458
  radA Streptococcus pneumoniae Rx1

49.057

93.392

0.458
  radA Streptococcus pneumoniae R6

49.057

93.392

0.458

Multiple sequence alignment