Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DKL56_RS05725 Genome accession   NZ_CP029476
Coordinates   1177818..1179194 (-) Length   458 a.a.
NCBI ID   WP_046307452.1    Uniprot ID   A0A3M0NHC6
Organism   Lactobacillus apis strain ESL0185     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1172818..1184194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKL56_RS05705 (DKL56_05705) rlmB 1173487..1174242 (-) 756 WP_109716047.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DKL56_RS05710 (DKL56_05710) - 1174229..1174672 (-) 444 WP_109715720.1 Mini-ribonuclease 3 -
  DKL56_RS05715 (DKL56_05715) cysS 1174665..1176089 (-) 1425 WP_109715721.1 cysteine--tRNA ligase -
  DKL56_RS05720 (DKL56_05720) gltX 1176237..1177742 (-) 1506 WP_074426298.1 glutamate--tRNA ligase -
  DKL56_RS05725 (DKL56_05725) radA 1177818..1179194 (-) 1377 WP_046307452.1 DNA repair protein RadA Machinery gene
  DKL56_RS05730 (DKL56_05730) - 1179195..1179743 (-) 549 WP_046307454.1 dUTP diphosphatase -
  DKL56_RS05735 (DKL56_05735) - 1179943..1180251 (+) 309 WP_109715722.1 hypothetical protein -
  DKL56_RS05740 (DKL56_05740) pepC 1180397..1181743 (+) 1347 WP_046307458.1 aminopeptidase C -
  DKL56_RS05745 (DKL56_05745) galT 1181831..1183309 (-) 1479 WP_109715723.1 UDP-glucose--hexose-1-phosphate uridylyltransferase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49977.82 Da        Isoelectric Point: 7.1405

>NTDB_id=293029 DKL56_RS05725 WP_046307452.1 1177818..1179194(-) (radA) [Lactobacillus apis strain ESL0185]
MAKIKTKYKCHSCGYISASYLGRCPNCGAWNQFEKETETVQAHSAKGSPSRLIKKTGINEPVKIEAVKAEKEERIQTSMG
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSELSQKFKVLYVSGEESANQIKLRADRLGLAANDMLLYPETDMEDI
RQQIDTIKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHAYRILHSVKNRFGAANEIGMFEMVNKGLQEVTNPSAIFLDERLPNSTGSAIVVSLEGTRPILAEIQALVTPTA
FGYAKRTTAGIDFNRAGLLLAVLEKRGNLMLQNQDVYLTATGGIRLNEPAVDLAVVMAIASSYKNQEILPTDCFVGEVGL
TGEVRRVNQIDARIKEAAKTGFKRIFIPKHNMRSSLQNMGIEVIPVSSIPQALKLVLA

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=293029 DKL56_RS05725 WP_046307452.1 1177818..1179194(-) (radA) [Lactobacillus apis strain ESL0185]
ATGGCCAAAATTAAAACGAAATATAAGTGTCACTCTTGTGGCTATATTTCGGCTAGTTACTTGGGAAGATGTCCTAATTG
CGGCGCCTGGAATCAGTTCGAAAAAGAAACCGAAACTGTCCAAGCGCATTCTGCAAAAGGTAGTCCAAGTAGGCTAATTA
AGAAAACTGGAATTAATGAACCAGTTAAAATTGAAGCCGTAAAGGCTGAAAAAGAGGAACGAATCCAGACAAGCATGGGT
GAACTTAACCGTGTTCTTGGTGGTGGAATAGTTCCTGGCTCGCTTGTGTTAATTGGTGGAGACCCTGGAATTGGTAAGTC
TACTTTAATGTTACAAATCATGAGTGAGCTATCCCAAAAGTTCAAAGTCCTATACGTTTCGGGAGAAGAATCGGCTAATC
AAATCAAGTTGCGCGCAGATCGTCTAGGACTCGCTGCGAATGATATGTTGCTTTATCCAGAAACAGACATGGAAGACATT
CGCCAGCAGATTGACACAATTAAGCCTGATTTTGTCGTAATTGACTCAATTCAAACAATGAATGAACCTAGTCTTGATTC
AATGACGGGTTCCGCATCACAGGTTCGTGAAGTAACCAGCGAATTAATGAAAATTGCTAAGATGGATGCAATTACGGTTT
TCGTGATCGGTCATGTTACCAAGGAAGGTGCAATTGCTGGGCCTAAGATTTTGGAGCACATGGTGGATACAGTTCTTTAT
TTTGAAGGTGATGAACACCATGCATACCGAATTTTGCATTCCGTAAAAAATCGGTTTGGTGCTGCTAACGAAATCGGTAT
GTTTGAAATGGTCAACAAAGGGTTGCAGGAAGTCACTAATCCATCTGCAATTTTTCTTGATGAGCGATTACCTAACTCAA
CGGGATCTGCGATTGTCGTCTCGCTTGAAGGAACAAGGCCAATTTTAGCGGAAATTCAAGCATTAGTTACACCGACTGCA
TTTGGCTATGCCAAAAGAACTACTGCCGGTATTGATTTCAACCGAGCAGGATTATTGTTAGCAGTTTTAGAAAAGCGTGG
CAACTTGATGCTACAAAATCAAGATGTTTATCTAACTGCTACCGGTGGTATTCGCTTGAATGAACCTGCGGTTGATTTGG
CGGTAGTGATGGCGATTGCTTCAAGTTATAAGAACCAAGAAATTTTACCAACTGATTGTTTCGTGGGTGAGGTTGGTTTA
ACTGGTGAAGTTAGACGAGTTAACCAGATTGATGCGCGGATTAAGGAAGCAGCTAAAACAGGGTTTAAGCGAATTTTTAT
TCCTAAGCATAATATGCGCTCAAGCTTGCAAAATATGGGAATAGAAGTAATTCCTGTTTCAAGCATCCCCCAAGCGTTAA
AATTGGTTTTGGCTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M0NHC6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

59.607

100

0.596
  radA Streptococcus mitis NCTC 12261

59.389

100

0.594
  radA Streptococcus pneumoniae Rx1

59.389

100

0.594
  radA Streptococcus pneumoniae D39

59.389

100

0.594
  radA Streptococcus pneumoniae R6

59.389

100

0.594
  radA Streptococcus pneumoniae TIGR4

59.389

100

0.594
  radA Bacillus subtilis subsp. subtilis str. 168

55.991

100

0.561

Multiple sequence alignment