Detailed information NTG0292982

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: DKK89_RS03925
• Genome accession: NZ_CP029474
• Coordinates: 746809..748158 (-)
• Length: 449 a.a.
• NCBI ID: WP_000815646.1
• Uniprot ID: Q9XB96
• Organism: Staphylococcus aureus strain USA 100 isolate 30-47
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	726820..748158	746809..748158	within	0

Gene organization within MGE regions

Location: 726820..748158

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DKK89_RS03805 (DKK89_03800)	mecA	726820..728829 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
DKK89_RS03810 (DKK89_03805)	mecR1	728926..730683 (+)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
DKK89_RS03815 (DKK89_03810)	mecI	730683..731054 (+)	372	WP_000369214.1	mecA-type methicillin resistance repressor MecI	-
DKK89_RS03820 (DKK89_03815)	psm-mec	731139..731207 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
DKK89_RS03825 (DKK89_03820)	-	731562..732671 (+)	1110	WP_000943131.1	ROK family transcriptional regulator	-
DKK89_RS03835 (DKK89_03830)	cstB	732785..734119 (-)	1335	Protein_716	persulfide dioxygenase-sulfurtransferase CstB	-
DKK89_RS03840 (DKK89_03835)	-	734152..735216 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
DKK89_RS03845 (DKK89_03840)	cstR	735352..735612 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
DKK89_RS03850 (DKK89_03845)	-	735612..736250 (+)	639	Protein_719	sulfite exporter TauE/SafE family protein	-
DKK89_RS03860 (DKK89_03855)	-	736576..737247 (-)	672	WP_127161440.1	class I SAM-dependent methyltransferase	-
DKK89_RS03875 (DKK89_03870)	erm(A)	737766..738497 (+)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
DKK89_RS03880 (DKK89_03875)	ant(9)	738621..739402 (-)	782	Protein_722	aminoglycoside nucleotidyltransferase ANT(9)	-
DKK89_RS03885 (DKK89_03880)	-	739563..739929 (-)	367	Protein_723	DUF6262 family protein	-
DKK89_RS03890 (DKK89_03885)	-	739936..741830 (-)	1895	Protein_724	site-specific integrase	-
DKK89_RS03895 (DKK89_03890)	-	741827..742912 (-)	1086	Protein_725	tyrosine-type recombinase/integrase	-
DKK89_RS03900 (DKK89_03895)	-	743031..743348 (-)	318	WP_000659050.1	JAB domain-containing protein	-
DKK89_RS03905 (DKK89_03900)	-	743369..743875 (-)	507	WP_001092169.1	DUF1643 domain-containing protein	-
DKK89_RS03910 (DKK89_03905)	-	743893..744204 (-)	312	WP_000700853.1	DUF960 family protein	-
DKK89_RS03915 (DKK89_03910)	-	744291..744641 (-)	351	WP_000859155.1	SAUGI family uracil-DNA glycosylase inhibitor	-
DKK89_RS03920 (DKK89_03915)	ccrB	745159..746787 (-)	1629	WP_001186602.1	recombinase family protein	Machinery gene
DKK89_RS03925 (DKK89_03920)	ccrA	746809..748158 (-)	1350	WP_000815646.1	recombinase family protein	Machinery gene

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52607.20 Da
• Isoelectric Point: 9.9585

>NTDB_id=292982 DKK89_RS03925 WP_000815646.1 746809..748158(-) (ccrA) [Staphylococcus aureus strain USA 100 isolate 30-47]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNTTLTNMTVRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CKDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=292982 DKK89_RS03925 WP_000815646.1 746809..748158(-) (ccrA) [Staphylococcus aureus strain USA 100 isolate 30-47]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATACAACACTTACGAATATGACCGTTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCTCGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCAAAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-250)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-322)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: Q9XB96

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	100	100	1
ccrA	Staphylococcus aureus COL	74.833	100	0.748