Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BPUM_RS11295 Genome accession   NC_009848
Coordinates   2276002..2276772 (-) Length   256 a.a.
NCBI ID   WP_012010612.1    Uniprot ID   A8FFB2
Organism   Bacillus pumilus SAFR-032     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2271002..2281772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS11275 (BPUM_2256) - 2271095..2271916 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  BPUM_RS11280 (BPUM_2257) - 2271942..2272580 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  BPUM_RS11285 (BPUM_2258) glyS 2272747..2274792 (-) 2046 WP_012010610.1 glycine--tRNA ligase subunit beta -
  BPUM_RS11290 (BPUM_2259) glyQ 2274785..2275672 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  BPUM_RS11295 (BPUM_2260) recO 2276002..2276772 (-) 771 WP_012010612.1 DNA repair protein RecO Machinery gene
  BPUM_RS11300 (BPUM_2261) - 2276808..2276951 (-) 144 WP_003217288.1 YqzL family protein -
  BPUM_RS11305 (BPUM_2262) era 2277101..2278006 (-) 906 WP_012010613.1 GTPase Era -
  BPUM_RS11310 (BPUM_2263) - 2277999..2278397 (-) 399 WP_012010614.1 cytidine deaminase -
  BPUM_RS11315 (BPUM_04185) - 2278511..2278897 (-) 387 WP_041815743.1 diacylglycerol kinase family protein -
  BPUM_RS11320 (BPUM_2264) ybeY 2278875..2279354 (-) 480 WP_012010615.1 rRNA maturation RNase YbeY -
  BPUM_RS11325 (BPUM_2265) - 2279355..2281466 (-) 2112 WP_371869448.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29230.90 Da        Isoelectric Point: 8.4348

>NTDB_id=29288 BPUM_RS11295 WP_012010612.1 2276002..2276772(-) (recO) [Bacillus pumilus SAFR-032]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=29288 BPUM_RS11295 WP_012010612.1 2276002..2276772(-) (recO) [Bacillus pumilus SAFR-032]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTGATGGCAAGAGGCGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGTGAAATGATTGAAAGTATGCGGACC
ATTCGGGAAGATTTATTTTTGACCGCATATGCCGCATACATGACAGAATTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCTTGCTTCAATCTCTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTTAAAATGTTATCAGTGATGGGGATGAAGCCCGAACTTGATCAATGTGTTCATTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCAATTAGAGATAACGGCTTCATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAAA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGACGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAAATGGAATCCATGAAAAAGCTCATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FFB2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754

Multiple sequence alignment