Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BPUM_RS08470 Genome accession   NC_009848
Coordinates   1740782..1741162 (+) Length   126 a.a.
NCBI ID   WP_225970079.1    Uniprot ID   -
Organism   Bacillus pumilus SAFR-032     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1735782..1746162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS08460 (BPUM_1680) - 1736274..1739417 (+) 3144 WP_012010090.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  BPUM_RS08465 (BPUM_1681) - 1739612..1740520 (+) 909 WP_041815555.1 trypsin-like serine peptidase -
  BPUM_RS08470 (BPUM_1682) nucA/comI 1740782..1741162 (+) 381 WP_225970079.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BPUM_RS08475 (BPUM_1683) - 1741301..1742149 (+) 849 WP_012010093.1 STAS domain-containing protein -
  BPUM_RS08480 (BPUM_1684) - 1742181..1742723 (-) 543 WP_012010094.1 IseA DL-endopeptidase inhibitor family protein -
  BPUM_RS08485 (BPUM_1685) - 1743018..1743452 (+) 435 WP_012010095.1 hypothetical protein -
  BPUM_RS08490 (BPUM_1686) lexA 1743498..1744118 (-) 621 WP_012010096.1 transcriptional repressor LexA -
  BPUM_RS08495 (BPUM_1687) yneA 1744278..1744589 (+) 312 WP_012010097.1 cell division suppressor protein YneA -
  BPUM_RS08500 (BPUM_1688) - 1744607..1745254 (+) 648 WP_012010098.1 YneB family resolvase-like protein -
  BPUM_RS08505 (BPUM_1689) - 1745324..1745554 (+) 231 WP_003211509.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13849.47 Da        Isoelectric Point: 7.3831

>NTDB_id=29281 BPUM_RS08470 WP_225970079.1 1740782..1741162(+) (nucA/comI) [Bacillus pumilus SAFR-032]
MGTLLSGFGEEHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCFEGGKGASVQYVSPSDNRGAGSWVGHQMSGYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=29281 BPUM_RS08470 WP_225970079.1 1740782..1741162(+) (nucA/comI) [Bacillus pumilus SAFR-032]
ATGGGCACGCTGTTAAGTGGATTTGGGGAGGAACATGCAGCAAAAGGTGCGGATCGATATGATCATGTCATTCAATTTCC
AAAAGAACGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGTGCAGATGCCCGCAGGCAAGAATCTTTAAAAGGCATTCCGACTAAACCAGGATTCGACCGAGATGAGTGGCCA
ATGGCCGTTTGTTTTGAAGGAGGAAAAGGGGCGAGTGTTCAATATGTCAGCCCTTCTGATAATAGGGGAGCAGGCTCATG
GGTTGGACACCAGATGAGTGGGTATCCTGATGGGAAAAGAATATTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment