Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   BPUM_RS07610 Genome accession   NC_009848
Coordinates   1563186..1564088 (+) Length   300 a.a.
NCBI ID   WP_012009946.1    Uniprot ID   A8FD75
Organism   Bacillus pumilus SAFR-032     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1558186..1569088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS07590 (BPUM_1505) - 1558852..1560588 (+) 1737 WP_012009943.1 hypothetical protein -
  BPUM_RS07595 (BPUM_1506) - 1560585..1560860 (+) 276 WP_012009944.1 FlhB-like flagellar biosynthesis protein -
  BPUM_RS07600 (BPUM_1507) sucC 1561037..1562197 (+) 1161 WP_012009945.1 ADP-forming succinate--CoA ligase subunit beta -
  BPUM_RS07605 (BPUM_1508) sucD 1562220..1563122 (+) 903 WP_003212080.1 succinate--CoA ligase subunit alpha -
  BPUM_RS07610 (BPUM_1509) dprA 1563186..1564088 (+) 903 WP_012009946.1 DNA-processing protein DprA Machinery gene
  BPUM_RS07615 (BPUM_1510) topA 1564282..1566357 (+) 2076 WP_012009947.1 type I DNA topoisomerase -
  BPUM_RS07620 (BPUM_1511) trmFO 1566428..1567732 (+) 1305 WP_012009948.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  BPUM_RS07625 (BPUM_1512) xerC 1567795..1568712 (+) 918 WP_012009949.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34192.64 Da        Isoelectric Point: 8.8799

>NTDB_id=29277 BPUM_RS07610 WP_012009946.1 1563186..1564088(+) (dprA) [Bacillus pumilus SAFR-032]
MYNVSERMIFHRLKGLISPSLLTKWWKVDPELYINEETHHFKQDRSLQTIDFTRLKQAEEKEFPIFQHIVQAYLKQNIHM
IPITSPMYPNTLKHIYDPPPVLFLKGNISYLNEEKSLGVVGTRVPSSYGEACVKKIVGELVKENWMIVSGLAKGIDGLAH
KECIRNKGKTIGIIAGGFQHLYPKEHVQMAEYMGEHHLLLSEHPPYVKPEKWHFPLRNRLISALTSGTIVIQCKEKSGSL
ITAYQALEQGKEVFAVAGSIFDPNSTGPARLIQQGAKLVHSTKDILEEFSFRSVQYTELL

Nucleotide


Download         Length: 903 bp        

>NTDB_id=29277 BPUM_RS07610 WP_012009946.1 1563186..1564088(+) (dprA) [Bacillus pumilus SAFR-032]
ATGTACAACGTGTCCGAAAGAATGATTTTTCACCGCTTAAAAGGCCTCATCTCACCCTCTTTGTTAACAAAATGGTGGAA
AGTCGATCCTGAGCTATATATAAATGAAGAAACACATCATTTCAAACAGGATCGATCATTACAAACAATCGACTTCACCC
GCCTAAAACAAGCCGAAGAAAAAGAATTCCCCATTTTTCAACACATCGTTCAAGCCTATTTAAAGCAAAACATTCACATG
ATTCCCATCACATCACCCATGTATCCCAACACACTAAAACATATTTATGATCCTCCCCCTGTGTTATTCCTAAAAGGAAA
CATATCGTATTTAAATGAAGAAAAAAGTTTAGGTGTAGTAGGCACACGAGTTCCATCGTCTTATGGAGAAGCATGTGTGA
AGAAAATTGTTGGTGAACTTGTAAAGGAAAATTGGATGATTGTCAGTGGCTTAGCAAAAGGCATTGATGGACTTGCACAT
AAAGAGTGCATAAGAAATAAAGGGAAAACGATTGGTATAATAGCAGGCGGATTTCAGCACTTATACCCAAAGGAACATGT
GCAAATGGCTGAATACATGGGCGAGCATCATTTGCTTTTGTCTGAGCATCCACCTTATGTCAAGCCAGAAAAGTGGCATT
TTCCTTTGAGGAATCGTTTAATTAGTGCACTCACAAGTGGAACCATCGTGATTCAGTGCAAAGAAAAGAGCGGTTCACTC
ATTACTGCCTATCAAGCACTTGAGCAAGGCAAAGAGGTATTTGCCGTTGCAGGATCAATCTTTGATCCTAATTCTACAGG
TCCAGCCAGACTTATACAGCAGGGAGCTAAGCTTGTTCATTCAACGAAGGATATTTTAGAGGAATTCTCCTTCCGCAGTG
TTCAATATACTGAACTCTTATAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FD75

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

54.704

95.667

0.523
  dprA Lactococcus lactis subsp. cremoris KW2

38.542

96

0.37

Multiple sequence alignment