Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   DKG76_RS06070 Genome accession   NZ_CP029465
Coordinates   1207642..1211346 (+) Length   1234 a.a.
NCBI ID   WP_003239417.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain KCTC 13429     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1202642..1216346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKG76_RS06055 (DKG76_06055) - 1203087..1203416 (-) 330 WP_003239420.1 four-helix bundle copper-binding protein -
  DKG76_RS06060 (DKG76_06060) - 1203535..1203972 (-) 438 WP_003239419.1 ferritin family protein -
  DKG76_RS06065 (DKG76_06065) addB 1204155..1207655 (+) 3501 WP_003239418.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  DKG76_RS06070 (DKG76_06070) addA 1207642..1211346 (+) 3705 WP_003239417.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  DKG76_RS06075 (DKG76_06075) sbcD 1211418..1212593 (+) 1176 WP_003239416.1 exonuclease subunit SbcD -
  DKG76_RS06080 (DKG76_06080) sbcC 1212590..1215982 (+) 3393 WP_003239414.1 exonuclease subunit SbcC -
  DKG76_RS06085 (DKG76_06085) - 1215996..1216295 (+) 300 Protein_1132 HNH endonuclease -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141119.43 Da        Isoelectric Point: 5.0687

>NTDB_id=292714 DKG76_RS06070 WP_003239417.1 1207642..1211346(+) (addA) [Bacillus inaquosorum strain KCTC 13429]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDQLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENDKGEQEPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGSEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDMNKSYLLDKELGFGTKFIHPQLRISFPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQATASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLEALAENGAGAHADISGHPARFAVQMIHSYD
LLDDDLEERLEEKSERLEAIRRGEPVPGPFAFDEKARKQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAEQTVHKLYEKELLTEEQKAAIDIEEIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=292714 DKG76_RS06070 WP_003239417.1 1207642..1211346(+) (addA) [Bacillus inaquosorum strain KCTC 13429]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATTACCGCAGAAGAAAACC
CGATTGATGTAGACCAGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGTTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGAGATGAAGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCTCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTATATGATGTATCGGAAAAGAGCGCAATCG
AAGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCGAAGGAGAAGCTGCAGCGCGCG
CTTGAACTGACGAAAGCGCCGGGCGGACCGGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCACTGAGCTTTATAAGCGGGTGCCCGCAGTTTCTTTTAAGCGAGCGAAAGCAGTAAAAG
GTGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACGGATTTGAGAAATGGCGCAAAAAAACTTCTCGAAAAGCTCAAA
ACCGACTACTTTACGAGAAGCCCTGAACAGCATTTGAAAAGCTTAGCCGACATGAAGCCTGTCATTGAAACACTCGTACA
GCTTGTCATCAGCTACGGAAAACGATTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCAGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAACGACAAAGGCGAACAGGAGCCGAGCGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTACAGGAATCGATCCTGCAGCTCGTCACAAGCGGATC
GGAAGAGACCGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCAGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGACGGAAAATCGACCTGAATAAGAATTTCCGAAGC
CGGGCTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTACCCGGACAATGATGAGACGGAAACAGAGCTGCTGCTGATCGACAACTCAG
AAGAGACGGATGCCGGCGAGGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGT
AAGTTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
ATTGTTACGTTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCAGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCGATTGTCGGGGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCGTACTATGAAGCGATGAAAGACTACTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACGT
TCTACGGACATCTGCAAAAATGGCGTGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAT
ACAAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGCGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTTGAA
TTTCCAGTCGTGTTTGCAGCGGGTCTCGGACGGAACTTCAACATGATGGACATGAACAAATCGTACCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTTTATTCATCCGCAATTGCGCATCAGCTTTCCGACGCTTCCGCTCATTGCCATGAAGAAAA
AAATGCGCAGGGAGCTATTGTCAGAGGAATTGCGTGTTCTCTATGTCGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCAACTGCGTCCCAAACTGACTGGCTTCTCCCGGA
ATTTGACCGCTATCAGGCAAAAACCTATTTAGATTTCATTGGACCTGCTCTTGCCAGACACAGAGACTTGGAGGCTTTGG
CTGAAAACGGTGCGGGAGCGCATGCTGACATATCAGGCCACCCTGCGCGTTTTGCCGTTCAAATGATCCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGATTGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCC
CGGCCCGTTTGCGTTTGATGAGAAAGCCCGGAAGCAGCTAAGCTGGACATATCCGCATCATGAGGTGACGCAAATTCGCA
CAAAGCAGTCAGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCCGTTAAACCGGCT
GACGGAAGCATTCTGTACAGGCGTCCCGCTTTTATGATGAAAAAAGGACTGACAGCGGCGGAAAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTCACACATGTGCCGACAATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAGCTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTTCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGTGCTAAATGGAAGGACCGGGAAGTGCCATTCAGTTTAGCGCTTCCGGCTAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAGGACGGTTTATATCTCTTAG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGTTTTGAAGGAGCGGCCCCGATCTTGAAAAAACGATACGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.759

100

0.968

Multiple sequence alignment