Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   DKE43_RS07695 Genome accession   NZ_CP029464
Coordinates   1467400..1468170 (-) Length   256 a.a.
NCBI ID   WP_127687053.1    Uniprot ID   -
Organism   Bacillus pumilus strain ZB201701     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1462400..1473170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKE43_RS07675 (DKE43_07705) - 1462491..1463312 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  DKE43_RS07680 (DKE43_07710) - 1463338..1463976 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  DKE43_RS07685 (DKE43_07715) glyS 1464143..1466188 (-) 2046 WP_106039267.1 glycine--tRNA ligase subunit beta -
  DKE43_RS07690 (DKE43_07720) glyQ 1466181..1467068 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  DKE43_RS07695 (DKE43_07725) recO 1467400..1468170 (-) 771 WP_127687053.1 DNA repair protein RecO Machinery gene
  DKE43_RS07700 (DKE43_07730) - 1468206..1468349 (-) 144 WP_003217288.1 YqzL family protein -
  DKE43_RS07705 (DKE43_07735) era 1468498..1469403 (-) 906 WP_060596663.1 GTPase Era -
  DKE43_RS07710 (DKE43_07740) - 1469396..1469794 (-) 399 WP_034661347.1 cytidine deaminase -
  DKE43_RS07715 (DKE43_07745) - 1469908..1470294 (-) 387 WP_066030644.1 diacylglycerol kinase family protein -
  DKE43_RS07720 (DKE43_07750) ybeY 1470272..1470751 (-) 480 WP_012010615.1 rRNA maturation RNase YbeY -
  DKE43_RS07725 (DKE43_07755) - 1470752..1472839 (-) 2088 WP_060596703.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29244.92 Da        Isoelectric Point: 8.4348

>NTDB_id=292665 DKE43_RS07695 WP_127687053.1 1467400..1468170(-) (recO) [Bacillus pumilus strain ZB201701]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELIDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYEEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=292665 DKE43_RS07695 WP_127687053.1 1467400..1468170(-) (recO) [Bacillus pumilus strain ZB201701]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGCGTGATGGCAAGAGGTGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTTCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGAACC
ATTCGGGAGGATTTATTTTTGACCGCATATGCCGCATATATGACAGAGTTAATCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTCTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTAATGGGAATGAAGCCTGAACTTGATCAATGTGTCCATTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCTATTAGAGATAACGGCTTCATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGAAGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.781

100

0.758

Multiple sequence alignment