Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DKE43_RS04820 Genome accession   NZ_CP029464
Coordinates   939358..939738 (+) Length   126 a.a.
NCBI ID   WP_060596689.1    Uniprot ID   -
Organism   Bacillus pumilus strain ZB201701     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 934358..944738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKE43_RS04810 (DKE43_04825) - 936555..937907 (+) 1353 WP_127686897.1 IS1182 family transposase -
  DKE43_RS04815 (DKE43_04830) - 938181..939089 (+) 909 WP_095285213.1 trypsin-like serine protease -
  DKE43_RS04820 (DKE43_04835) nucA/comI 939358..939738 (+) 381 WP_060596689.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DKE43_RS04825 (DKE43_04840) - 939886..940734 (+) 849 WP_060596420.1 STAS domain-containing protein -
  DKE43_RS04830 (DKE43_04845) - 940768..941310 (-) 543 WP_127686962.1 IseA DL-endopeptidase inhibitor family protein -
  DKE43_RS04835 (DKE43_04850) - 941605..942042 (+) 438 WP_034660769.1 hypothetical protein -
  DKE43_RS04840 (DKE43_04855) lexA 942090..942710 (-) 621 WP_003212311.1 transcriptional repressor LexA -
  DKE43_RS04845 (DKE43_04860) yneA 942870..943181 (+) 312 WP_003211955.1 cell division suppressor protein YneA -
  DKE43_RS04850 (DKE43_04865) - 943199..943846 (+) 648 WP_003211322.1 recombinase family protein -
  DKE43_RS04855 (DKE43_04870) - 943916..944146 (+) 231 WP_034660771.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13796.47 Da        Isoelectric Point: 8.4984

>NTDB_id=292659 DKE43_RS04820 WP_060596689.1 939358..939738(+) (nucA/comI) [Bacillus pumilus strain ZB201701]
MGTLLGGFGEKHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISSYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=292659 DKE43_RS04820 WP_060596689.1 939358..939738(+) (nucA/comI) [Bacillus pumilus strain ZB201701]
ATGGGCACGTTGTTAGGTGGATTTGGGGAGAAACATGCGGCAAAAGGTGCGGATCGATATGATCATGTCATTCAATTTCC
AAAGGAACGGTACCCTGAAACAGGCAGCCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACTAAACCTGGCTTTGACCGGGATGAGTGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGTGCGAGTGTTCAATATGTCAGCCCATCTGATAATAGGGGAGCAGGCTCATG
GGTTGGACACCAGATTAGCAGTTATCCTGATGGGAAAAGAATTTTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment