Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DKB62_RS10665 Genome accession   NZ_CP029462
Coordinates   2248798..2250174 (+) Length   458 a.a.
NCBI ID   WP_107196637.1    Uniprot ID   A0A346B1J4
Organism   Megasphaera stantonii strain AJH120     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2243798..2255174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKB62_RS10645 (DKB62_10645) - 2244752..2245969 (-) 1218 WP_087476936.1 nucleotidyltransferase family protein -
  DKB62_RS10650 (DKB62_10650) - 2246192..2246731 (+) 540 WP_232818840.1 hypothetical protein -
  DKB62_RS10655 (DKB62_10655) - 2246760..2247956 (+) 1197 WP_087476938.1 acetate/propionate family kinase -
  DKB62_RS10660 (DKB62_10660) - 2248098..2248787 (+) 690 WP_107196636.1 HAD family hydrolase -
  DKB62_RS10665 (DKB62_10665) radA 2248798..2250174 (+) 1377 WP_107196637.1 DNA repair protein RadA Machinery gene
  DKB62_RS10670 (DKB62_10670) - 2250347..2251540 (+) 1194 WP_087476941.1 PIN/TRAM domain-containing protein -
  DKB62_RS10675 (DKB62_10675) ispD 2251541..2252731 (+) 1191 WP_232818841.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DKB62_RS10680 (DKB62_10680) gltX 2252754..2254217 (+) 1464 WP_107196638.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49331.14 Da        Isoelectric Point: 8.4739

>NTDB_id=292623 DKB62_RS10665 WP_107196637.1 2248798..2250174(+) (radA) [Megasphaera stantonii strain AJH120]
MAKTKIRYVCANCGSVSSRWLGRCPQCGEWNTMMEEQAVPEPPKGVGARQGKGAKPALLQDIAMEQMSRVRTGIGELDRV
LGGGIVPGALILLSGDPGIGKSTLVLQIADAVCRHAGAVLYGSGEESAGQIKVRAERLGITASNLIIQADTSLDAVLQEA
KRRRPVLLIIDSIQTMYSSDADGMPGSMTQIRESTRRLMEFAKTESISVIVIGHVTKEGAIAGPRMMEHMVDVVLYFEGE
RNYQFRVLRGIKNRFGSTNETGLFTMEETGLSELTNPSQMLLAERSANQAGSAVAAVMDGMRPLLGEIQALTTHSVFAVP
RRTASGMDYNRLIILLAVLEKRVGLSLGTQDVYINSVGGLRVTETAADLAVVLAVFSSLRDVPMDSRTVVMGEVGLTGDI
RRVPHALRRIKEGVKMGFQTFIIPKGNLDEIKEGAFPQCRIIGAATLRDAIQAAFTAT

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=292623 DKB62_RS10665 WP_107196637.1 2248798..2250174(+) (radA) [Megasphaera stantonii strain AJH120]
ATGGCTAAAACGAAGATCCGATACGTATGCGCTAACTGCGGCAGCGTGTCCAGCCGCTGGCTGGGCCGGTGCCCCCAGTG
CGGAGAATGGAATACGATGATGGAAGAACAGGCTGTGCCTGAACCGCCTAAAGGCGTCGGCGCGCGGCAGGGCAAGGGGG
CGAAGCCGGCCTTGCTGCAGGATATCGCCATGGAACAGATGAGCCGCGTCCGGACGGGCATCGGCGAATTGGACCGCGTG
CTGGGCGGCGGCATCGTGCCGGGAGCGCTGATCTTGCTGAGCGGCGACCCCGGTATCGGCAAATCGACCTTGGTGCTGCA
GATTGCCGATGCCGTGTGCCGCCATGCCGGCGCGGTGCTGTACGGCTCCGGCGAAGAATCGGCCGGACAGATTAAGGTGC
GGGCGGAACGATTGGGAATTACGGCTTCCAATCTCATCATACAGGCCGATACGTCTCTCGACGCCGTACTGCAGGAAGCA
AAGCGACGCCGTCCGGTGCTGCTGATCATCGATTCTATCCAGACGATGTACAGCAGCGACGCCGACGGCATGCCGGGCAG
CATGACTCAGATACGGGAAAGCACGCGGCGTCTCATGGAATTTGCCAAGACGGAGTCCATTTCGGTCATCGTCATAGGCC
ATGTCACGAAGGAAGGGGCGATTGCCGGACCGCGCATGATGGAACATATGGTAGACGTCGTCCTGTATTTTGAAGGCGAG
CGGAATTATCAGTTCCGCGTGCTGCGGGGAATCAAAAATCGCTTTGGGTCGACGAACGAAACGGGATTGTTTACGATGGA
AGAAACGGGGTTGTCCGAATTGACTAATCCGTCGCAAATGCTCCTGGCCGAGCGGTCGGCAAATCAAGCCGGCTCGGCTG
TCGCCGCCGTCATGGACGGCATGCGGCCCCTTTTAGGGGAGATTCAAGCCCTGACAACGCATTCCGTTTTTGCCGTCCCG
CGCCGCACGGCGTCGGGCATGGACTATAACCGGCTGATTATTCTGCTGGCCGTGCTGGAAAAGCGCGTCGGCCTGTCTTT
GGGCACGCAGGACGTGTATATCAACAGCGTCGGCGGCCTGCGCGTCACGGAAACTGCCGCCGATTTAGCCGTCGTGCTGG
CCGTTTTTTCCAGCCTGCGCGACGTGCCGATGGACAGCCGAACCGTCGTCATGGGTGAAGTAGGCCTGACCGGCGACATA
CGGCGCGTGCCCCATGCCTTGCGGCGCATCAAGGAAGGCGTGAAGATGGGGTTCCAGACCTTTATCATTCCCAAGGGAAA
TCTGGATGAAATCAAGGAAGGAGCTTTCCCGCAATGCCGGATTATCGGGGCGGCGACGCTGCGCGACGCGATACAGGCGG
CGTTTACAGCGACGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(350-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A346B1J4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.584

100

0.487
  radA Streptococcus pneumoniae Rx1

48.465

99.563

0.483
  radA Streptococcus pneumoniae D39

48.465

99.563

0.483
  radA Streptococcus pneumoniae R6

48.465

99.563

0.483
  radA Streptococcus pneumoniae TIGR4

48.465

99.563

0.483
  radA Streptococcus mitis NCTC 12261

48.352

99.345

0.48
  radA Streptococcus mitis SK321

48.352

99.345

0.48

Multiple sequence alignment