Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BPUM_RS01760 Genome accession   NC_009848
Coordinates   332806..333234 (-) Length   142 a.a.
NCBI ID   WP_012008879.1    Uniprot ID   A8F9U2
Organism   Bacillus pumilus SAFR-032     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 327806..338234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS01730 (BPUM_0306) - 327963..328292 (+) 330 WP_012008873.1 YckD family protein -
  BPUM_RS01735 (BPUM_0307) - 328340..329104 (-) 765 WP_012008874.1 MBL fold metallo-hydrolase -
  BPUM_RS01740 (BPUM_0308) - 329220..329636 (+) 417 WP_012008875.1 Lrp/AsnC family transcriptional regulator -
  BPUM_RS01745 (BPUM_0309) - 329666..330472 (+) 807 WP_012008876.1 aminoglycoside N(3)-acetyltransferase -
  BPUM_RS01750 (BPUM_0310) - 330474..330860 (-) 387 WP_012008877.1 competence protein ComJ -
  BPUM_RS01755 (BPUM_0311) - 330981..332678 (-) 1698 WP_012008878.1 methyl-accepting chemotaxis protein -
  BPUM_RS01760 (BPUM_0312) nucA/comI 332806..333234 (-) 429 WP_012008879.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BPUM_RS01770 (BPUM_0314) - 333602..335671 (+) 2070 WP_012008881.1 hydantoinase/oxoprolinase family protein -
  BPUM_RS01775 (BPUM_0315) - 335661..337646 (+) 1986 WP_012008882.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15880.69 Da        Isoelectric Point: 4.8200

>NTDB_id=29256 BPUM_RS01760 WP_012008879.1 332806..333234(-) (nucA/comI) [Bacillus pumilus SAFR-032]
MKLLKIILLLLLIVVGVATGYIQLEQSKQETTNSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSVCTIDREHSDEQREQS
LQGTPTKRGYDRDEWPMAMCKEGGTGASVKYISPSDNRGAGSWVGHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=29256 BPUM_RS01760 WP_012008879.1 332806..333234(-) (nucA/comI) [Bacillus pumilus SAFR-032]
ATGAAACTTCTTAAAATCATTCTTTTGCTTTTATTGATCGTCGTTGGTGTAGCAACAGGCTATATTCAGCTGGAGCAAAG
TAAACAAGAGACGACGAATTCATCTTATGACAAGACAATTCACTTCCCATCAGATCGTTACCCCGAGACAGCGAAGCACA
TAGAGGAGGCAATTGACGAAGGACATTCATCTGTATGTACGATTGACCGTGAACATAGCGATGAACAAAGAGAACAATCA
CTTCAAGGCACCCCAACAAAGCGCGGGTATGATCGAGATGAATGGCCGATGGCGATGTGCAAGGAAGGAGGAACAGGAGC
CTCAGTCAAGTATATTAGTCCTTCAGATAATAGAGGAGCAGGCTCATGGGTCGGTCATCAATTATCTGATGATCCCGACG
GCACAAGAATACAATTTATCATCGATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8F9U2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

74.528

74.648

0.556

Multiple sequence alignment