Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BPUM_RS00535 Genome accession   NC_009848
Coordinates   104253..105632 (+) Length   459 a.a.
NCBI ID   WP_034620767.1    Uniprot ID   A0AAE3WNQ6
Organism   Bacillus pumilus SAFR-032     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 99253..110632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS00515 (BPUM_0068) - 99595..100059 (+) 465 WP_003217184.1 CtsR family transcriptional regulator -
  BPUM_RS00520 (BPUM_0069) - 100074..100631 (+) 558 WP_041815104.1 UvrB/UvrC motif-containing protein -
  BPUM_RS00525 (BPUM_0070) - 100636..101727 (+) 1092 WP_012008685.1 protein arginine kinase -
  BPUM_RS00530 (BPUM_0071) clpC 101724..104159 (+) 2436 WP_003216969.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BPUM_RS00535 (BPUM_0072) radA 104253..105632 (+) 1380 WP_034620767.1 DNA repair protein RadA Machinery gene
  BPUM_RS00540 (BPUM_0073) disA 105635..106714 (+) 1080 WP_012008686.1 DNA integrity scanning diadenylate cyclase DisA -
  BPUM_RS00545 (BPUM_0074) - 106868..107968 (+) 1101 WP_012008687.1 PIN/TRAM domain-containing protein -
  BPUM_RS00550 (BPUM_0075) ispD 107982..108671 (+) 690 WP_012008688.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BPUM_RS00555 (BPUM_0076) ispF 108675..109151 (+) 477 WP_012008689.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50024.54 Da        Isoelectric Point: 7.9305

>NTDB_id=29255 BPUM_RS00535 WP_034620767.1 104253..105632(+) (radA) [Bacillus pumilus SAFR-032]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSDEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIKSTSLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=29255 BPUM_RS00535 WP_034620767.1 104253..105632(+) (radA) [Bacillus pumilus SAFR-032]
ATGGCTAAGACGAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGTAAAGAGCCAGCAAACCGTCGAAGCGCTTTTAACCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCAGATGAACCCCGAATCAAAACGAATTTAGAAGAATTT
AATCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTCATTGGCGGAGATCCTGGGATTGGGAAGTCCACTTT
ATTATTACAAGTATCAGCACAGCTCTCAGATAAAAATCAGAATGTATTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTCGGCATAAAAAGCACCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAAGAGATGAAACCCGCTTTTGTCGTGGTGGATTCGATTCAAACCGTTTATCAAAGCGATATTACGTCAGC
TCCTGGGTCTGTATCTCAAGTGAGAGAATGTACAGCACAGCTGATGAAGATTGCAAAGACAAATGGGATTCCAATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGCCCACGTCTTTTAGAGCATATGGTGGACACGGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATCTTACGTGCGGTGAAAAACCGATTTGGCTCGACGAATGAACTAGGGATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCCGAAATTTTCTTAGAAGAACGTTCGGCAGGTGTATCGG
GTTCGTGTGTCGTTGCCTCAATGGAAGGAACAAGACCTGTTCTAGTCGAGATACAAGCATTAATTTCACCGACAAGCTTC
GGAAATCCGCGTAGAATGGCCACTGGTCTTGATCATAATCGAGTGTCACTACTCATGGCGGTTTTAGAAAAACGTGTTGG
ACTGCTTCTGCAAAACCAGGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCAGCAATTGACTTGGCCA
TTGCGGTCAGCATTGCATCAAGCTTTAAAGACGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGCCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCGAAACTAGGATTTAAACGAATGTTTATTCC
TCAGGCAAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTCGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.118

99.782

0.869
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608

Multiple sequence alignment