Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DJ572_RS01525 Genome accession   NZ_CP029461
Coordinates   281127..281576 (-) Length   149 a.a.
NCBI ID   WP_017696127.1    Uniprot ID   -
Organism   Bacillus subtilis strain QB61     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 276127..286576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ572_RS01490 (DJ572_01490) yckA 276402..277082 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  DJ572_RS01495 (DJ572_01495) yckB 277092..277955 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  DJ572_RS01500 - 278117..278309 (+) 193 Protein_273 hypothetical protein -
  DJ572_RS01505 (DJ572_01500) - 278345..278665 (+) 321 Protein_274 RDD family protein -
  DJ572_RS01510 (DJ572_01505) yckD 278747..279079 (+) 333 WP_015252849.1 YckD family protein -
  DJ572_RS01515 (DJ572_01510) bglC 279232..280665 (+) 1434 WP_119899001.1 6-phospho-beta-glucosidase -
  DJ572_RS01520 (DJ572_01515) nin/comJ 280702..281100 (-) 399 WP_017696126.1 DNA-entry nuclease inhibitor Regulator
  DJ572_RS01525 (DJ572_01520) nucA/comI 281127..281576 (-) 450 WP_017696127.1 DNA-entry nuclease Machinery gene
  DJ572_RS01530 (DJ572_01525) tlpC 281744..283465 (-) 1722 WP_119899003.1 methyl-accepting chemotaxis protein TlpC -
  DJ572_RS01535 (DJ572_01530) hxlB 283576..284133 (-) 558 WP_038428343.1 6-phospho-3-hexuloisomerase -
  DJ572_RS01540 (DJ572_01535) hxlA 284139..284771 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  DJ572_RS01545 (DJ572_01540) hxlR 285004..285366 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16412.34 Da        Isoelectric Point: 4.5282

>NTDB_id=292539 DJ572_RS01525 WP_017696127.1 281127..281576(-) (nucA/comI) [Bacillus subtilis strain QB61]
MNITTDIIKTILLVIVIIAAAAAGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=292539 DJ572_RS01525 WP_017696127.1 281127..281576(-) (nucA/comI) [Bacillus subtilis strain QB61]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGCCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGAT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment