Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DFH04_RS02930 Genome accession   NZ_CP029458
Coordinates   650823..652184 (-) Length   453 a.a.
NCBI ID   WP_003376641.1    Uniprot ID   A0A9Q4XVK2
Organism   Clostridium novyi strain 150557     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 645823..657184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DFH04_RS02910 (DFH04_02910) ispD 647201..647887 (-) 687 WP_003375958.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DFH04_RS02915 (DFH04_02915) - 647908..648987 (-) 1080 WP_003375783.1 PIN/TRAM domain-containing protein -
  DFH04_RS02920 (DFH04_02920) - 649291..649692 (+) 402 WP_003376909.1 hypothetical protein -
  DFH04_RS02925 (DFH04_02925) disA 649729..650793 (-) 1065 WP_003375526.1 DNA integrity scanning diadenylate cyclase DisA -
  DFH04_RS02930 (DFH04_02930) radA 650823..652184 (-) 1362 WP_003376641.1 DNA repair protein RadA Machinery gene
  DFH04_RS02935 (DFH04_02935) - 652347..654788 (-) 2442 WP_039235782.1 ATP-dependent Clp protease ATP-binding subunit -
  DFH04_RS02940 (DFH04_02940) - 654813..655847 (-) 1035 WP_003380740.1 protein arginine kinase -
  DFH04_RS02945 (DFH04_02945) - 655866..656384 (-) 519 WP_003375111.1 UvrB/UvrC motif-containing protein -
  DFH04_RS02950 (DFH04_02950) - 656454..656912 (-) 459 WP_003380738.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50066.19 Da        Isoelectric Point: 7.5448

>NTDB_id=292517 DFH04_RS02930 WP_003376641.1 650823..652184(-) (radA) [Clostridium novyi strain 150557]
MAKSKNVFICQECGYEAPKWFGKCPGCATWNSMVEEKKMDTIKSKRVGITQNNSKPESILNIKSGEFNRYDTGIVELNRV
LGGGLVKGSLTLISGSPGIGKSTILLQTANNIASKVGKVLYVSGEESGEQIKIRGDRIGNISPELYILSETNLELIEEHI
NYMEPVFIIIDSIQTLFKPSIESAPGSVSQVRECSNELMRIAKTKNIPLFIVAHVTKQGELAGPRVLEHMVDTVLSFEGE
RTQEFRILRTLKNRFGTTSEIGVFEMREEGLKEIFNPSEVFLEETNFNLEGSIVIGIMEGTRPILVEIQALVTETKAIMP
RRTAVGVDNSRLNLILAVLEKKLKIPFYNCDVYVNVVGGIDIEGTYGDLGLALALISSIKGRNFKLDKMIVFGEIGLTGE
VRPISLCDRLVNEGIKMGFENIVIPTRNKEKIITKNKNVIGVSSLKEAVSKVF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=292517 DFH04_RS02930 WP_003376641.1 650823..652184(-) (radA) [Clostridium novyi strain 150557]
ATGGCTAAGAGCAAAAATGTTTTTATATGCCAAGAATGTGGGTATGAGGCTCCTAAATGGTTTGGAAAATGTCCAGGTTG
TGCTACTTGGAATAGTATGGTTGAAGAGAAAAAGATGGATACTATAAAGAGTAAGAGGGTTGGAATAACTCAAAACAATA
GTAAGCCTGAGAGTATATTAAATATTAAATCAGGAGAATTTAATAGATATGATACTGGCATTGTAGAATTAAATAGAGTA
CTTGGGGGAGGGCTTGTTAAAGGATCTTTAACGTTAATATCAGGGTCGCCAGGTATAGGTAAATCCACAATATTGTTACA
AACAGCTAATAATATTGCATCTAAAGTAGGTAAAGTATTATATGTGTCAGGTGAAGAATCAGGAGAACAAATAAAAATAA
GAGGGGATAGAATAGGGAACATATCTCCAGAATTATATATTTTATCTGAAACTAATTTGGAATTAATAGAAGAACATATT
AATTATATGGAACCTGTATTTATTATTATAGATTCCATACAGACATTATTTAAACCATCTATAGAATCAGCACCAGGTAG
TGTATCACAAGTTAGAGAGTGCTCTAATGAACTTATGAGAATTGCAAAAACAAAAAATATACCTTTATTTATAGTTGCCC
ATGTAACTAAGCAGGGGGAACTTGCAGGACCTAGAGTGTTAGAGCATATGGTAGATACGGTTCTTTCTTTTGAAGGTGAA
AGAACACAAGAGTTTAGAATATTAAGAACATTAAAAAATAGATTTGGTACTACAAGTGAAATTGGAGTATTTGAAATGAG
AGAAGAAGGGCTTAAGGAAATATTCAATCCATCCGAAGTATTTCTAGAGGAGACTAATTTTAATTTAGAGGGATCTATAG
TAATAGGTATTATGGAAGGAACTCGCCCTATTTTAGTTGAAATACAAGCTTTAGTGACGGAAACTAAAGCCATCATGCCA
AGAAGAACAGCTGTAGGTGTGGACAATTCTAGGTTAAACTTGATATTAGCAGTGTTAGAGAAAAAGTTAAAAATACCTTT
TTACAACTGTGATGTTTATGTTAATGTAGTTGGTGGCATAGATATTGAGGGTACATATGGAGACTTAGGTCTTGCTCTAG
CTTTGATATCAAGCATTAAAGGAAGAAATTTCAAACTTGATAAAATGATAGTTTTTGGAGAAATAGGATTAACAGGAGAA
GTAAGACCTATTTCATTATGTGATAGACTCGTTAATGAAGGTATAAAGATGGGATTCGAAAATATTGTTATACCTACTAG
AAATAAGGAAAAAATTATAACTAAAAATAAGAATGTTATTGGAGTATCATCATTAAAAGAAGCAGTGAGTAAAGTATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-428)

AAA domain (AAA_25)

(60-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

52.967

100

0.532
  radA Streptococcus mitis NCTC 12261

50.769

100

0.51
  radA Streptococcus pneumoniae D39

50.441

100

0.506
  radA Streptococcus pneumoniae TIGR4

50.441

100

0.506
  radA Streptococcus pneumoniae R6

50.441

100

0.506
  radA Streptococcus pneumoniae Rx1

50.441

100

0.506
  radA Streptococcus mitis SK321

50.22

100

0.503

Multiple sequence alignment