Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   BPUM_RS00020 Genome accession   NC_009848
Coordinates   3057..4169 (+) Length   370 a.a.
NCBI ID   WP_012008631.1    Uniprot ID   A8F8Y7
Organism   Bacillus pumilus SAFR-032     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS00005 (BPUM_0001) dnaA 1..1341 (+) 1341 WP_012008628.1 chromosomal replication initiator protein DnaA -
  BPUM_RS00010 (BPUM_0002) dnaN 1538..2674 (+) 1137 WP_041815072.1 DNA polymerase III subunit beta -
  BPUM_RS00015 (BPUM_0003) yaaA 2825..3040 (+) 216 WP_041815077.1 S4 domain-containing protein YaaA -
  BPUM_RS00020 (BPUM_0004) recF 3057..4169 (+) 1113 WP_012008631.1 DNA replication/repair protein RecF Machinery gene
  BPUM_RS00025 (BPUM_0005) remB 4187..4432 (+) 246 WP_041815080.1 extracellular matrix regulator RemB -
  BPUM_RS00030 (BPUM_0006) gyrB 4490..6406 (+) 1917 WP_041816108.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  BPUM_RS00035 (BPUM_0007) gyrA 6639..9158 (+) 2520 WP_012008634.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42554.62 Da        Isoelectric Point: 7.3979

>NTDB_id=29251 BPUM_RS00020 WP_012008631.1 3057..4169(+) (recF) [Bacillus pumilus SAFR-032]
MYIQSLALTSYRNYEHTELQFDNKVNVMIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDQDYAKIEGRVIKKNGP
LPMQLVISKKGKKGKVNHIEQQKLSHYVGALNTIMFAPEDLSLVKGSPQIRRRFLDMEIGQVSAVYLHDLSLYQKILSQR
NHYLKQLQTRKQTDQAMLEVLTEQLIDAAAKVVKRRLTFTKQLEKWAQPLHFGISRELETLTLQYHTAIEVSEASDLSKI
KNSYEESFQKLRDREIDRGVTLWGPHRDDLLFFVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVEGIDHATLKEAEIFRVASGKVID

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=29251 BPUM_RS00020 WP_012008631.1 3057..4169(+) (recF) [Bacillus pumilus SAFR-032]
ATGTACATTCAAAGTCTGGCGTTAACTTCATACCGAAACTATGAACACACCGAGCTTCAATTCGACAACAAGGTGAATGT
CATGATCGGTGAGAATGCCCAAGGTAAAACGAACTTGATGGAAGCGATCTATGTATTGTCGATGGCAAAGTCGCATCGTA
CGTCAAATGATAAAGAACTTATCCGATGGGACCAAGACTATGCTAAAATAGAAGGTAGAGTCATCAAAAAAAATGGTCCA
CTCCCAATGCAACTCGTGATTTCAAAAAAAGGGAAAAAGGGCAAGGTCAATCACATTGAACAACAGAAGCTCAGTCATTA
TGTTGGTGCGCTAAACACCATCATGTTTGCACCAGAGGACTTAAGTCTTGTAAAGGGCAGCCCGCAAATCCGCAGAAGAT
TCCTCGATATGGAGATTGGACAAGTTTCTGCTGTCTACTTGCATGATTTATCGCTCTATCAAAAAATTCTCTCTCAGCGG
AATCATTACTTGAAACAATTGCAGACTAGAAAGCAAACGGATCAAGCGATGCTGGAGGTTTTAACAGAGCAATTGATTGA
TGCGGCAGCGAAGGTTGTCAAAAGACGACTGACTTTTACGAAACAGCTCGAAAAATGGGCGCAGCCGTTGCATTTTGGCA
TTTCTAGAGAGCTAGAAACACTCACGCTCCAATACCACACGGCGATAGAGGTATCAGAAGCGTCAGACTTGTCGAAAATA
AAGAATAGCTATGAAGAATCGTTTCAGAAACTAAGAGACAGAGAAATAGACCGAGGAGTGACGCTGTGGGGACCTCACAG
AGATGACCTACTTTTCTTTGTGAATGGTCGGGATGTTCAGACATATGGCTCTCAAGGGCAACAAAGAACAACAGCTCTAT
CACTTAAGCTGGCAGAAATCGACTTGATACATGAAGAAATCGGAGAATATCCCATTCTTTTACTCGATGATGTTTTATCT
GAACTTGATGATTACAGACAGTCTCATTTGCTCCATACCATTCAGGGACGTGTACAGACCTTCGTCACTACAACAAGTGT
TGAAGGCATCGATCACGCCACCTTGAAAGAAGCGGAAATTTTCAGAGTAGCCAGTGGAAAAGTAATTGACTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8F8Y7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

84.011

99.73

0.838

Multiple sequence alignment