Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EQK40_RS00525 Genome accession   NZ_CP035123
Coordinates   104595..105263 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain EC25     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 99595..110263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQK40_RS00500 (EQK40_00500) pyrE 100120..100761 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EQK40_RS00505 (EQK40_00505) - 101059..102018 (+) 960 WP_000340183.1 reverse transcriptase family protein -
  EQK40_RS00510 (EQK40_00510) slmA 102064..102660 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EQK40_RS00515 (EQK40_00515) dut 102767..103225 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EQK40_RS00520 (EQK40_00520) coaBC 103203..104423 (-) 1221 WP_000050158.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EQK40_RS00525 (EQK40_00525) radC 104595..105263 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EQK40_RS00530 (EQK40_00530) rpmB 105480..105716 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EQK40_RS00535 (EQK40_00535) rpmG 105737..105904 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EQK40_RS00540 (EQK40_00540) mutM 106002..106811 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EQK40_RS00545 (EQK40_00545) coaD 106850..107329 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  EQK40_RS00550 (EQK40_00550) waaA 107337..108614 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EQK40_RS00555 (EQK40_00555) rfaQ 109027..110085 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=292407 EQK40_RS00525 WP_001297375.1 104595..105263(+) (radC) [Escherichia coli strain EC25]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=292407 EQK40_RS00525 WP_001297375.1 104595..105263(+) (radC) [Escherichia coli strain EC25]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGTTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGACTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496