Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DJ533_RS15545 Genome accession   NZ_CP029397
Coordinates   2738823..2739626 (-) Length   267 a.a.
NCBI ID   WP_065994418.1    Uniprot ID   A0A2S2FG11
Organism   Acinetobacter defluvii strain WCHA30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2733823..2744626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ533_RS15525 (DJ533_15560) - 2734867..2735568 (-) 702 WP_065994422.1 tetratricopeptide repeat protein -
  DJ533_RS15530 (DJ533_15565) hisS 2735597..2736892 (-) 1296 WP_065994421.1 histidine--tRNA ligase -
  DJ533_RS15535 (DJ533_15570) ispG 2736896..2738017 (-) 1122 WP_065994420.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DJ533_RS15540 (DJ533_15575) - 2738041..2738832 (-) 792 WP_065994419.1 RodZ domain-containing protein -
  DJ533_RS15545 (DJ533_15580) pilF 2738823..2739626 (-) 804 WP_065994418.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DJ533_RS15550 (DJ533_15585) rlmN 2739671..2740906 (-) 1236 WP_065994417.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  DJ533_RS15555 (DJ533_15590) ndk 2741075..2741506 (-) 432 WP_016166212.1 nucleoside-diphosphate kinase -
  DJ533_RS15560 (DJ533_15595) iscX 2741625..2741825 (-) 201 WP_065994416.1 Fe-S cluster assembly protein IscX -
  DJ533_RS15565 (DJ533_15600) - 2741922..2742473 (-) 552 WP_065994415.1 phosphatase PAP2 family protein -
  DJ533_RS15570 (DJ533_15605) - 2742503..2743792 (-) 1290 WP_065994414.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30076.18 Da        Isoelectric Point: 8.8442

>NTDB_id=292235 DJ533_RS15545 WP_065994418.1 2738823..2739626(-) (pilF) [Acinetobacter defluvii strain WCHA30]
MYQKTVAIAAICCAALLTQACQTTQTGSVLKKDPEKAVKVRTQLAAEYIRTNDLDAAKRSLDQALEIDAGDSTANMMMGV
LLQQEGSKISMDKADGYFKRAISRDPDNAQARNNYGTYLYLSKRYNDAIEQFKIAGASLGYDQRYKALENLGRVYLLTGD
SENAEKTFKQALQVNRDSVISLIELSELLYLKQQNALASQLYEQFVRLVGQKNQDPRALWIGIRIARANGDQMGSQVLVN
QLRALFPESQQYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=292235 DJ533_RS15545 WP_065994418.1 2738823..2739626(-) (pilF) [Acinetobacter defluvii strain WCHA30]
ATGTACCAAAAAACAGTCGCAATTGCAGCGATCTGTTGCGCTGCGTTGTTGACACAAGCATGTCAAACGACACAAACTGG
ATCGGTTTTGAAAAAAGATCCTGAAAAAGCAGTGAAAGTGCGAACTCAATTGGCTGCTGAATATATTCGGACCAATGATC
TTGATGCGGCAAAACGTTCACTAGATCAAGCCTTGGAAATAGACGCTGGTGACTCCACTGCAAATATGATGATGGGGGTT
TTGCTGCAACAAGAAGGCAGTAAAATCAGCATGGATAAAGCAGATGGATATTTTAAACGTGCTATTTCTCGTGATCCAGA
CAATGCTCAAGCCCGTAATAACTATGGGACTTACTTATATTTATCGAAACGTTATAATGATGCCATTGAACAATTCAAGA
TTGCAGGTGCAAGTTTGGGTTATGACCAACGCTATAAAGCGTTGGAAAATCTTGGCAGAGTTTATTTGTTGACTGGGGAT
AGTGAAAATGCTGAAAAAACATTCAAGCAAGCCCTGCAAGTCAATCGAGATTCGGTTATCTCTTTAATCGAGTTGTCAGA
ACTATTATATTTAAAACAACAAAATGCTCTAGCAAGCCAGTTGTATGAGCAATTTGTTCGTTTAGTGGGTCAAAAAAATC
AAGATCCAAGAGCGCTTTGGATCGGAATTCGTATCGCAAGAGCGAATGGCGATCAGATGGGATCGCAAGTATTGGTTAAT
CAACTACGTGCTTTATTCCCTGAAAGTCAACAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(146-177)

Tetratricopeptide repeat (TPR_8)

(40-69)

Tetratricopeptide repeat (TPR_17)

(98-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2FG11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

74.609

95.88

0.715
  pilF Acinetobacter baylyi ADP1

65.53

98.876

0.648

Multiple sequence alignment