Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   DIC78_RS04275 Genome accession   NZ_CP029364
Coordinates   846575..850279 (-) Length   1234 a.a.
NCBI ID   WP_127695512.1    Uniprot ID   -
Organism   Bacillus halotolerans strain ZB201702     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 841575..855279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DIC78_RS04260 (DIC78_04275) - 841620..841922 (-) 303 WP_024120842.1 hypothetical protein -
  DIC78_RS04265 (DIC78_04280) sbcC 841936..845328 (-) 3393 WP_127695510.1 exonuclease subunit SbcC -
  DIC78_RS04270 (DIC78_04285) sbcD 845325..846503 (-) 1179 WP_106022371.1 exonuclease subunit SbcD -
  DIC78_RS04275 (DIC78_04290) addA 846575..850279 (-) 3705 WP_127695512.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  DIC78_RS04280 (DIC78_04295) addB 850266..853766 (-) 3501 WP_127695514.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  DIC78_RS04285 (DIC78_04300) - 853950..854387 (+) 438 WP_044156482.1 ferritin family protein -
  DIC78_RS04290 (DIC78_04305) - 854516..854830 (+) 315 WP_044156484.1 four-helix bundle copper-binding protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141634.14 Da        Isoelectric Point: 5.1301

>NTDB_id=291954 DIC78_RS04275 WP_127695512.1 846575..850279(-) (addA) [Bacillus halotolerans strain ZB201702]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLDGAKEKLQRA
LELTKAPGGPAPRAENFLDDLAQIDDLIQHQDDFNELYQRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHVKSLADMEPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENNETETELLLIDKSEEADAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALTEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGTAAVSDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAFEEKARERLAWKYPHREVTQIRTKQSVSEIKRRREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAERTVQHLYEKELLTEEQQSAINIEEIVQFFHTEIGE
KLIGAKWKNREVPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTRAVEQIAQTKVRGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=291954 DIC78_RS04275 WP_127695512.1 846575..850279(-) (addA) [Bacillus halotolerans strain ZB201702]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGCTCCGGTAAAACCGCTGTTCTTGTCGAAAGAATGATCCGGAAAATCACGGCGGAAGAAAATC
CGATTGATGTAGACCGGCTTCTCGTTGTCACCTTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCA
CTGGAAAAAGAGCTGGTGAAACGCCCAGGATCACTGCATATCCGACGCCAGCTGTCGCTGTTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTACCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGTGATAAGGCG
TTCTTTGAGCTTGTCGACCGCTACACGACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTACGAGTA
TTCCCGTTCCCATCCTGATCCGGAGACATGGCTGAAAAGCTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCGTATGTCAAAGAAGATATTGCGATGGTGCTGGACGGAGCAAAGGAAAAGCTGCAGCGTGCC
CTTGAGCTGACAAAAGCGCCGGGCGGACCTGCGCCGCGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATCAACGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGACGAGTTCGATCCCGTCTTGCTTGATGAAGCGACAGACTTAAGAAATGGCGCCAAAAAACTGCTTGAAAAACTTAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATGTAAAAAGTTTGGCTGACATGGAGCCTGTCATTGAAACACTCGTCCA
GCTTGTTATCAGCTACGGGAAACGGTTTGAAGCCGCGAAGCAGGAGAAATCGCTTATCGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAAGGCGAACGTGAGCCGAGTGAGGCGGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTTGTCGATGAATATCAGGATACCAACCTGGTGCAAGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TTCTTTCAAAGTACAAACGGTTTACAGAGAGCGGAGAAGGTACTGGCCGGAAAATCGACTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTCTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTAGATTATGATGA
ACAGGCTGAGCTGAAACTCGGAGCGGCGTATCCGGAAAACAACGAAACGGAAACAGAGCTGCTGCTGATTGACAAGTCAG
AGGAGGCGGACGCAGGCGAGGAAGCGGAAGAGCTGGAAACGGTGCAGTTCGAAGCTAAAGCGATCGCTAAAGAAATACGC
AAGCTGATCTCATCGCCATTTAAAGTATATGACGGGAAAACGAAGACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCGATGCCGTGGGCGCCGCAAATCATGGAGGAACTGCGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGATACTTTGATGCGGTAGAAGTGTCTGTGGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAGCTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCGTACTATGAAGCAATGAAAGACTATCTGGCAGCCGGCGACCGAAACGATGAGCTCTATCAAAAGCTGCAAACGT
TTTACGGACATTTGCAAAAGTGGCGCGCTTTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCTAACTTGCGTGTCCTTTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGCTTCTTGAGGTTTATCGAACGCATGCAGGAACGGGGCGATG
ATCTGGGAACGGCGAGAGCGTTGACCGAACAGGAGGATGTTGTCCGTTTGATGACGATTCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTCTTCACAGCGGGACTCGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTACCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAACTGCTGTCTGAGGAATTGCGTGTCCTCTATGTTGCTTTAACGAGGGCAAAGGAAAAGCTGTTTCTT
GTAGGCTCATGCAAGGATCATCAGAAACAGCTGGCAAAATGGCAGGCATCCGCGTCCCAAGCTGACTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCAGCTCTTGTCAGACACAGAGACTTGGAGTCTTTAG
CTGAGGGCGGTACGGCAGCGGTTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATTCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGTGGTGAACCGGTTCC
CGGCCCGTTTGCATTTGAGGAAAAAGCTCGCGAGCGGCTGGCCTGGAAGTATCCGCATCGCGAGGTGACACAAATTCGCA
CGAAGCAGTCTGTTTCTGAGATTAAGAGAAGAAGAGAGTACGAAGACGAATACAGCGGACGGGCGCCCGTTAAACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAAAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGATAGAAGAAGCGGAGCGGACAGTGCAGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCAATATCGAGGAAATCGTTCAATTTTTCCATACGGAAATTGGCGAA
AAACTGATCGGCGCTAAATGGAAGAACCGCGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGATGGCTTATATTTGCTGG
ACTATAAAACAGACCGAATTGAGGGTAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAGAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

93.436

100

0.934

Multiple sequence alignment