Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DIW83_RS16775 Genome accession   NZ_CP029351
Coordinates   3453349..3454149 (-) Length   266 a.a.
NCBI ID   WP_004710659.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain NCTC 8102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3448349..3459149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DIW83_RS16755 (DIW83_16755) - 3449420..3450112 (-) 693 WP_016803730.1 YfgM family protein -
  DIW83_RS16760 (DIW83_16760) hisS 3450141..3451433 (-) 1293 WP_016803731.1 histidine--tRNA ligase -
  DIW83_RS16765 (DIW83_16765) ispG 3451430..3452545 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DIW83_RS16770 (DIW83_16770) - 3452567..3453358 (-) 792 WP_016803732.1 helix-turn-helix domain-containing protein -
  DIW83_RS16775 (DIW83_16775) pilF 3453349..3454149 (-) 801 WP_004710659.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DIW83_RS16780 (DIW83_16780) rlmN 3454165..3455397 (-) 1233 WP_002053401.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  DIW83_RS16785 (DIW83_16785) ndk 3455520..3455951 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  DIW83_RS16790 (DIW83_16790) iscX 3456065..3456265 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  DIW83_RS16795 (DIW83_16795) - 3456365..3456916 (-) 552 WP_016803733.1 phosphatase PAP2 family protein -
  DIW83_RS16800 (DIW83_16800) - 3456941..3458239 (-) 1299 WP_079744809.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30097.44 Da        Isoelectric Point: 9.2307

>NTDB_id=291908 DIW83_RS16775 WP_004710659.1 3453349..3454149(-) (pilF) [Acinetobacter nosocomialis strain NCTC 8102]
MSTPKLKIMLCMGVAVAFLASGCQSSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=291908 DIW83_RS16775 WP_004710659.1 3453349..3454149(-) (pilF) [Acinetobacter nosocomialis strain NCTC 8102]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCTGTTGCATTTCTGGCCAGTGGTTGTCAGTCTTCGCC
AACGCTTAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCCGCAGAATACATTCGTTCTGGTGACTTGG
ACTCTGCAAAGCGTTCCCTTGACCAAGCTTTAAGTGTCGATAGCCGTGATGCAACGGCAAATATGATGATGGGCATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCATCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCCATTGAGCAATTCCGTATTG
CTGGTGCAACATTAGGTTATGATCAACGCTATCAAGCCTTAGAAAATCTAGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGATTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.496

100

0.985
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729

Multiple sequence alignment