Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   TLET_RS01265 Genome accession   NC_009828
Coordinates   256897..257781 (-) Length   294 a.a.
NCBI ID   WP_012002296.1    Uniprot ID   A8F3T1
Organism   Pseudothermotoga lettingae TMO     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 251897..262781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TLET_RS01250 (Tlet_0242) - 253507..254496 (-) 990 WP_012002293.1 P1 family peptidase -
  TLET_RS01255 (Tlet_0243) - 254549..254980 (+) 432 WP_012002294.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  TLET_RS01260 (Tlet_0244) - 254977..256896 (+) 1920 WP_012002295.1 transglycosylase domain-containing protein -
  TLET_RS01265 (Tlet_0245) pilA 256897..257781 (-) 885 WP_012002296.1 signal recognition particle-docking protein FtsY Machinery gene
  TLET_RS01270 (Tlet_0246) - 257784..259511 (-) 1728 WP_012002297.1 proline--tRNA ligase -
  TLET_RS10755 (Tlet_0247) - 259596..261617 (+) 2022 WP_012002298.1 HD domain-containing phosphohydrolase -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 31880.81 Da        Isoelectric Point: 5.7556

>NTDB_id=29187 TLET_RS01265 WP_012002296.1 256897..257781(-) (pilA) [Pseudothermotoga lettingae TMO]
MGFFERLKTGLSRARKALFEGLSQILKGSKISDEILEELEERLLMADVGYPTTEYIIEQLKQSKTNDALETLKEIMIDLL
SFDSKLNSPDQPPFVITVVGVNGTGKTTTVAKLAAYFQSTGKTVVLGAADTFRAAAIEQLREWGERTGSTVIYHSEGADS
AAVAFDAVSHAKAREKDVVIIDTAGRLHTKKNLMEELKKVHRVIGKVVPGAPHEVLLVIDATTGQNGLVQAKVFKEMVDV
TGIVITKLDGTAKGGISLAIKKELNLPIKFIGIGEGAEDLKPFDAKEFVEALID

Nucleotide


Download         Length: 885 bp        

>NTDB_id=29187 TLET_RS01265 WP_012002296.1 256897..257781(-) (pilA) [Pseudothermotoga lettingae TMO]
ATGGGATTTTTCGAACGACTTAAAACAGGACTTTCCAGAGCAAGAAAAGCGCTGTTTGAAGGACTTTCACAAATTCTTAA
AGGTAGCAAAATAAGCGATGAAATTCTCGAAGAGCTTGAAGAAAGATTGCTGATGGCAGATGTCGGTTATCCAACAACAG
AATACATAATAGAGCAATTGAAACAGAGCAAAACCAATGATGCACTCGAAACTTTAAAAGAAATAATGATTGATCTGCTT
TCTTTCGATAGCAAGCTTAATTCACCAGATCAACCTCCCTTTGTGATAACAGTTGTTGGAGTTAATGGAACAGGTAAAAC
CACAACTGTAGCAAAACTCGCTGCGTATTTTCAATCAACTGGGAAAACAGTTGTATTGGGTGCAGCAGATACCTTCAGGG
CAGCAGCAATAGAACAATTAAGAGAATGGGGAGAAAGAACTGGCTCTACGGTTATTTATCATTCCGAAGGAGCTGATTCT
GCCGCAGTTGCTTTTGATGCTGTAAGCCATGCGAAGGCTCGAGAAAAAGATGTGGTGATAATCGATACAGCAGGAAGGCT
GCATACGAAAAAGAACCTTATGGAAGAACTGAAAAAAGTTCACAGAGTTATCGGTAAAGTTGTCCCGGGAGCACCTCACG
AGGTTTTGCTGGTCATAGATGCTACCACTGGGCAAAACGGATTGGTTCAAGCAAAGGTTTTCAAGGAAATGGTAGATGTA
ACTGGGATCGTTATAACAAAGCTTGATGGCACAGCAAAAGGCGGTATATCTTTAGCAATCAAAAAAGAATTAAATTTACC
TATCAAATTCATAGGAATAGGGGAAGGTGCCGAAGATCTTAAGCCATTCGACGCAAAAGAGTTTGTAGAAGCTTTAATTG
ATTGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(18-70)

SRP54-type protein, GTPase domain (SRP54)

(93-293)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8F3T1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.845

100

0.503

Multiple sequence alignment