Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HME7025_RS05815 Genome accession   NZ_CP029346
Coordinates   1314437..1315816 (-) Length   459 a.a.
NCBI ID   WP_109322725.1    Uniprot ID   A0A2S2DW50
Organism   Aquirufa nivalisilvae strain HME7025     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1309437..1320816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HME7025_RS05800 (HME7025_01151) - 1310765..1311346 (+) 582 WP_109322723.1 NTP transferase domain-containing protein -
  HME7025_RS05805 (HME7025_01152) purL 1311457..1313685 (+) 2229 WP_109325021.1 phosphoribosylformylglycinamidine synthase subunit PurL -
  HME7025_RS05810 (HME7025_01153) truA 1313698..1314465 (+) 768 WP_109322724.1 tRNA pseudouridine(38-40) synthase TruA -
  HME7025_RS05815 (HME7025_01154) radA 1314437..1315816 (-) 1380 WP_109322725.1 DNA repair protein RadA Machinery gene
  HME7025_RS05820 (HME7025_01155) - 1315921..1316601 (+) 681 WP_109322726.1 porin family protein -
  HME7025_RS05825 (HME7025_01156) pheS 1316617..1317642 (+) 1026 WP_109322727.1 phenylalanine--tRNA ligase subunit alpha -
  HME7025_RS05830 (HME7025_01157) - 1317711..1320689 (+) 2979 WP_109322728.1 DUF2723 domain-containing protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50613.32 Da        Isoelectric Point: 6.2681

>NTDB_id=291824 HME7025_RS05815 WP_109322725.1 1314437..1315816(-) (radA) [Aquirufa nivalisilvae strain HME7025]
MAKAKTSFICQSCGQSSPKWLGKCPACGEWNTFVEELLETTIHPAEVWKNKSTHRTAPVPKLLQEITFESSPKLQCSDEE
FNRVLGGGIVPGSLVLIGGEPGIGKSTLLLQVALSLQGPKILYVTGEESEQQIKLRADRLGPISQDLYILTETNTQQIFK
HLVDLEPDLIIIDSIQTLYSDQIESGPGSVSQIRECALELMRFAKESGTPIFLVGHITKEGSIAGPKVLEHLVDTVLQFE
GDRNMIYRILRTTKNRFGSTSELGIYEMLGNGLRPVSNPSEILLSSRDEPASGIAIGTILEGNRPLLIEIQSLVTQTSYA
TAQRTATGYDAKRLNMLLAVLEKRGGFRLGTQDVFLNITGGIRVDDPALDLAACMSLLSSHEDKVIDSSICFASEVGLGG
ELRAVTRIDQRIAEAEKLGFKEIWISKYNQKSINYKAKTIKVQLASTWMEVMMNIWKRK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=291824 HME7025_RS05815 WP_109322725.1 1314437..1315816(-) (radA) [Aquirufa nivalisilvae strain HME7025]
GTGGCCAAAGCCAAAACCTCTTTTATTTGTCAATCTTGTGGACAATCATCACCTAAATGGTTAGGGAAATGTCCAGCATG
CGGAGAATGGAACACTTTTGTGGAAGAACTCCTTGAAACTACCATTCATCCTGCTGAAGTTTGGAAAAATAAAAGCACTC
ATAGAACGGCTCCTGTACCGAAATTATTGCAAGAAATCACTTTTGAGAGCAGTCCAAAACTTCAATGTTCGGATGAAGAA
TTCAATCGGGTATTGGGTGGAGGTATTGTACCTGGAAGTTTAGTCCTGATTGGAGGAGAACCCGGAATTGGGAAATCTAC
TTTATTGCTTCAAGTAGCTCTATCACTTCAAGGCCCCAAAATATTGTATGTTACCGGAGAAGAATCCGAACAACAGATCA
AATTAAGAGCTGATCGTTTAGGACCCATCTCTCAAGATTTATACATCTTAACAGAAACAAATACTCAACAGATTTTCAAG
CATTTGGTTGACTTAGAGCCTGATTTAATCATTATTGATTCTATTCAAACCCTCTATTCTGATCAAATTGAATCCGGCCC
TGGTTCGGTGTCGCAAATTCGAGAATGTGCCTTGGAGCTCATGAGATTTGCCAAAGAAAGTGGCACGCCTATCTTTTTAG
TGGGGCATATTACAAAGGAAGGATCCATTGCTGGACCTAAAGTGTTAGAACATTTAGTCGATACTGTTCTTCAATTTGAA
GGTGATCGAAATATGATTTATCGTATTCTTCGTACTACCAAAAATCGATTTGGTAGTACTTCAGAATTGGGTATTTATGA
AATGTTGGGCAATGGCCTTAGACCTGTGAGTAATCCATCCGAAATCCTTCTTTCCTCTAGGGATGAACCTGCCTCAGGTA
TCGCTATTGGAACTATTTTGGAAGGAAACAGGCCACTTTTGATTGAAATTCAATCGTTAGTAACCCAAACATCTTATGCC
ACAGCTCAAAGAACAGCTACAGGATATGATGCCAAACGATTAAATATGTTGCTCGCCGTACTAGAGAAAAGAGGAGGATT
TAGGCTAGGAACGCAAGACGTATTTTTGAATATAACAGGAGGAATCAGAGTGGATGATCCTGCCTTGGATTTGGCAGCTT
GTATGTCTTTACTTTCTTCTCATGAAGATAAAGTCATAGATTCTAGCATTTGTTTCGCATCTGAAGTGGGATTGGGTGGA
GAACTTCGAGCGGTAACTCGTATCGATCAACGCATCGCTGAGGCTGAAAAACTGGGATTCAAAGAGATTTGGATCAGTAA
ATACAACCAAAAAAGCATCAATTATAAAGCCAAAACAATCAAAGTTCAATTGGCAAGCACCTGGATGGAAGTTATGATGA
ACATTTGGAAACGAAAGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(75-221)

Subunit ChlI of Mg-chelatase (ChlI)

(344-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2DW50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae R6

50.547

99.564

0.503
  radA Streptococcus pneumoniae Rx1

50.547

99.564

0.503
  radA Streptococcus pneumoniae D39

50.547

99.564

0.503
  radA Streptococcus pneumoniae TIGR4

50.547

99.564

0.503
  radA Streptococcus mitis NCTC 12261

50.547

99.564

0.503
  radA Streptococcus mitis SK321

50.328

99.564

0.501
  radA Bacillus subtilis subsp. subtilis str. 168

52.887

94.336

0.499

Multiple sequence alignment