Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DIE29_RS13770 Genome accession   NZ_CP029331
Coordinates   2887408..2888856 (+) Length   482 a.a.
NCBI ID   WP_102042875.1    Uniprot ID   -
Organism   Pseudothauera hydrothermalis strain GD-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2882408..2893856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DIE29_RS13740 thiL 2883115..2884074 (+) 960 WP_102042869.1 thiamine-phosphate kinase -
  DIE29_RS13745 - 2884076..2884555 (+) 480 WP_102042870.1 phosphatidylglycerophosphatase A -
  DIE29_RS13750 - 2884568..2885074 (+) 507 WP_102042871.1 CinA family protein -
  DIE29_RS13755 recA 2885186..2886220 (+) 1035 WP_102042872.1 recombinase RecA Machinery gene
  DIE29_RS13760 recX 2886230..2886685 (+) 456 WP_102042873.1 recombination regulator RecX -
  DIE29_RS13765 lptB 2886666..2887388 (+) 723 WP_102042874.1 LPS export ABC transporter ATP-binding protein -
  DIE29_RS13770 rpoN/rpoN1 2887408..2888856 (+) 1449 WP_102042875.1 RNA polymerase factor sigma-54 Machinery gene
  DIE29_RS13775 hpf 2888876..2889199 (+) 324 WP_102042876.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DIE29_RS13780 ptsN 2889333..2889818 (+) 486 WP_102042877.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DIE29_RS13785 hprK 2889787..2890725 (+) 939 WP_102042878.1 HPr(Ser) kinase/phosphatase -
  DIE29_RS13790 - 2890833..2892449 (+) 1617 WP_162860643.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 482 a.a.        Molecular weight: 53457.55 Da        Isoelectric Point: 5.4930

>NTDB_id=291731 DIE29_RS13770 WP_102042875.1 2887408..2888856(+) (rpoN/rpoN1) [Pseudothauera hydrothermalis strain GD-2]
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTIELNQEIERFLLENPMLERDDGEVGGLGATLPDRPAPGATETSAADGSDA
ARDDAPEQDPARYDEAGEWLSAAGSGSRRNDDDEEADFQEFHAAATSLRDHLDQQVALSPLSDRDRALVRFIIEALDDDG
YLNQSLEELLPLLPEALDFDLDDLNIALRHVQNLEPAGVGARSVQECLSLQLRALPAGATRDLALKIVEHHLALLAERNF
VKLKRLLSCDDEALRAAHALICRLDPHPGSRYAAQETRYIIPDVVVRKLHGQWTVSLNPEAVPKLRVNALYAQILQQHRG
NGGGLSSQLQEARWLIKNVQQRFDTILRVAQAIVDQQRQFFDHGEVAMRPLTLREIADQLGLHESTVSRVTTQKFMATPR
GVFELKYFFGSHVSTDTGGAASSTAIRALIRQLVDAEDRKKPLSDAKIAELLGQQGIVVARRTIAKYRESLNIPPVSLRK
TI

Nucleotide


Download         Length: 1449 bp        

>NTDB_id=291731 DIE29_RS13770 WP_102042875.1 2887408..2888856(+) (rpoN/rpoN1) [Pseudothauera hydrothermalis strain GD-2]
ATGAAACCCACTCTCCAGCTCAAGCTTTCGCAACATCTCACGCTCACCCCGCAACTGCAGCAGTCGATCAAACTGCTGCA
GTTATCCACCATCGAGCTGAACCAGGAGATCGAGCGCTTCCTGCTGGAAAACCCGATGCTGGAACGCGACGATGGGGAGG
TGGGCGGATTGGGCGCCACGCTGCCGGATCGCCCCGCGCCCGGCGCCACTGAAACCTCGGCGGCCGATGGCAGCGACGCG
GCCCGCGACGATGCGCCCGAACAAGACCCCGCACGCTACGATGAAGCTGGCGAATGGCTGAGCGCCGCGGGCAGCGGCAG
CCGCCGTAACGACGATGACGAAGAGGCCGATTTCCAGGAGTTCCATGCGGCGGCCACCTCGCTACGCGACCACCTCGATC
AACAGGTCGCACTCTCTCCGCTTTCGGACCGCGACCGCGCATTGGTGCGCTTCATCATCGAGGCGCTCGACGACGACGGC
TATCTGAACCAATCGCTGGAAGAACTGCTGCCGCTGCTGCCCGAAGCGCTGGATTTCGATCTGGACGATCTGAACATCGC
CCTGCGCCATGTGCAAAACCTGGAGCCGGCCGGCGTGGGCGCACGCAGTGTGCAAGAGTGCCTGAGCCTGCAACTGCGCG
CCTTGCCCGCGGGCGCCACCCGCGATCTGGCGCTCAAGATTGTGGAGCATCATTTGGCGCTGCTCGCCGAGCGCAATTTC
GTCAAACTGAAGCGGCTGTTGTCCTGTGACGATGAGGCGCTGCGTGCAGCCCATGCGCTGATCTGCCGGCTCGATCCGCA
TCCCGGCTCGCGCTACGCCGCGCAGGAAACCCGCTACATCATCCCGGATGTGGTCGTACGCAAACTGCATGGTCAATGGA
CGGTGAGCCTCAACCCCGAGGCGGTGCCCAAACTGCGGGTCAACGCCCTGTATGCGCAGATTCTGCAACAGCACCGTGGC
AACGGCGGCGGCCTGAGCAGTCAATTGCAAGAAGCGCGCTGGCTGATCAAAAACGTACAGCAGCGCTTCGACACCATCTT
GCGGGTGGCCCAAGCCATCGTTGACCAGCAGCGGCAGTTCTTCGATCATGGGGAGGTGGCCATGCGCCCGCTCACCCTGC
GCGAAATCGCAGACCAGTTGGGCTTGCATGAATCCACCGTGTCACGGGTCACCACCCAGAAATTCATGGCCACGCCCAGG
GGGGTGTTCGAACTCAAGTATTTTTTCGGCAGTCATGTCTCCACCGATACCGGTGGGGCGGCATCCTCCACGGCGATTCG
CGCGCTGATTCGCCAGCTGGTGGATGCCGAAGACAGAAAGAAGCCTTTGTCCGACGCCAAGATTGCCGAACTGTTGGGCC
AGCAGGGTATCGTCGTCGCACGGCGCACCATCGCCAAATACCGCGAATCGCTCAATATCCCGCCGGTCAGTCTGCGCAAA
ACAATTTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(326-480)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(118-311)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

56.287

100

0.585

Multiple sequence alignment