Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DG474_RS00135 Genome accession   NZ_CP029257
Coordinates   23623..24987 (+) Length   454 a.a.
NCBI ID   WP_185756299.1    Uniprot ID   -
Organism   Streptococcus oralis strain CCUG 53468     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 18623..29987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DG474_RS00105 (DG474_00100) comW 19965..20201 (+) 237 WP_242746665.1 sigma(X)-activator ComW -
  DG474_RS00110 (DG474_00105) - 20443..21729 (+) 1287 WP_255778237.1 adenylosuccinate synthase -
  DG474_RS00115 (DG474_00110) tadA 21930..22397 (+) 468 WP_084933671.1 tRNA adenosine(34) deaminase TadA -
  DG474_RS00125 (DG474_00120) - 22588..23031 (+) 444 WP_255778238.1 dUTP diphosphatase -
  DG474_RS00130 (DG474_00125) - 23095..23583 (+) 489 WP_061416456.1 hypothetical protein -
  DG474_RS00135 (DG474_00130) radA 23623..24987 (+) 1365 WP_185756299.1 DNA repair protein RadA Machinery gene
  DG474_RS00140 (DG474_00135) - 25060..25554 (+) 495 WP_002876632.1 carbonic anhydrase -
  DG474_RS00145 (DG474_00140) - 25768..26736 (+) 969 WP_000010175.1 ribose-phosphate diphosphokinase -
  DG474_RS00150 (DG474_00145) - 26857..27294 (+) 438 WP_000076968.1 CoA-binding protein -
  DG474_RS00155 (DG474_00150) - 27323..28333 (-) 1011 WP_076984873.1 YeiH family protein -
  DG474_RS00160 (DG474_00155) - 28485..29654 (+) 1170 WP_200370601.1 pyridoxal phosphate-dependent aminotransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49547.05 Da        Isoelectric Point: 6.1894

>NTDB_id=291384 DG474_RS00135 WP_185756299.1 23623..24987(+) (radA) [Streptococcus oralis strain CCUG 53468]
MIAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNR
VLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRSEV
ERIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGE
RHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNA
KRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEI
RRVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIEVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=291384 DG474_RS00135 WP_185756299.1 23623..24987(+) (radA) [Streptococcus oralis strain CCUG 53468]
ATCATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGTCCTAA
CTGTGGGTCTTGGTCTTCTTTTGTAGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGA
AAACCAAGCCCATGAAACTGGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGT
GTACTTGGAGGCGGAGTGGTACCAGGGAGTCTCGTCCTTATCGGTGGGGATCCAGGGATTGGGAAATCAACCCTTCTCTT
ACAAGTATCGACTCAGCTGTCCCAAGTAGGGACTGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTCC
GTGCAGAGCGCTTGGGGGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGATCTGAGGTG
GAGCGCATCCAACCAGATTTTCTCATCATCGACTCTATCCAGACGATTATGTCTCCTGAGATTTCAGGGGTGCAGGGGTC
TGTTTCTCAGGTGCGTGAGGTGACAGCTGAACTCATGCAGTTGGCTAAGACTAATAACATTGCCATCTTTATCGTAGGGC
ATGTGACCAAGGAAGGGACCTTGGCGGGTCCGCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAG
CGCCACCATACCTTCCGTATTTTGAGGGCAGTCAAAAACCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCA
GTCGGGTGGATTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAGGAGCGTTTAGATGGGGCTACTGGCTCGTCAA
TCGTTGTGACCATGGAAGGGACCCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCAACCATGTTTGGAAATGCT
AAACGCACGACGACAGGACTTGATTTCAATCGTGCAAGTCTGATTATGGCTGTTTTGGAAAAACGAGCAGGGCTTCTCTT
GCAAAATCAGGATGCCTATCTCAAATCTGCTGGTGGCGTGAAATTGGATGAGCCTGCTATTGACTTAGCCGTTGCAGTGG
CTATTGCCTCTAGTTACAAGGACAAGCCTACCAATCCTCAGGAATGTTTTGTGGGTGAACTGGGCCTGACTGGAGAAATT
CGGCGCGTGAATCGTATCGAACAACGTATCAATGAAGCGGCAAAACTGGGCTTTACCAAGATTTATGTACCCAAGAATTC
CTTGACAGGAATCACTCCACCCAAGGAAATTGAAGTCATTGGTGTGACAACGATTCAGGAAGTTTTGAAGAAGGTCTTTG
CATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-219)

Subunit ChlI of Mg-chelatase (ChlI)

(361-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.338

99.78

0.991
  radA Streptococcus mitis SK321

99.117

99.78

0.989
  radA Streptococcus pneumoniae Rx1

99.117

99.78

0.989
  radA Streptococcus pneumoniae D39

99.117

99.78

0.989
  radA Streptococcus pneumoniae R6

99.117

99.78

0.989
  radA Streptococcus pneumoniae TIGR4

99.117

99.78

0.989
  radA Bacillus subtilis subsp. subtilis str. 168

63.355

99.78

0.632

Multiple sequence alignment