Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   A1D75_RS26000 Genome accession   NZ_CP034806
Coordinates   5028023..5028691 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 2010C-3347     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5023023..5033691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1D75_RS25970 rfaQ 5023201..5024259 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  A1D75_RS25975 waaA 5024672..5025949 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  A1D75_RS25980 coaD 5025957..5026436 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  A1D75_RS25985 mutM 5026475..5027284 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  A1D75_RS25990 rpmG 5027382..5027549 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  A1D75_RS25995 rpmB 5027570..5027806 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  A1D75_RS26000 radC 5028023..5028691 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  A1D75_RS26005 coaBC 5028863..5030083 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  A1D75_RS26010 dut 5030061..5030519 (+) 459 WP_000976070.1 dUTP diphosphatase -
  A1D75_RS26015 slmA 5030626..5031222 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  A1D75_RS26020 pyrE 5031259..5031900 (-) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  A1D75_RS26025 rph 5031966..5032682 (-) 717 WP_001247093.1 ribonuclease PH -
  A1D75_RS26030 yicC 5032809..5033672 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=291342 A1D75_RS26000 WP_001297375.1 5028023..5028691(-) (radC) [Escherichia coli strain 2010C-3347]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=291342 A1D75_RS26000 WP_001297375.1 5028023..5028691(-) (radC) [Escherichia coli strain 2010C-3347]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496