Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CRG85_RS00340 Genome accession   NZ_CP034787
Coordinates   73073..73741 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain ECCNB20-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 68073..78741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRG85_RS00310 (CRG85_00310) yicC 68093..68956 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  CRG85_RS00315 (CRG85_00315) rph 69083..69799 (+) 717 WP_001247093.1 ribonuclease PH -
  CRG85_RS00320 (CRG85_00320) pyrE 69864..70505 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CRG85_RS00325 (CRG85_00325) slmA 70542..71138 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CRG85_RS00330 (CRG85_00330) dut 71245..71703 (-) 459 WP_000976070.1 dUTP diphosphatase -
  CRG85_RS00335 (CRG85_00335) coaBC 71681..72901 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CRG85_RS00340 (CRG85_00340) radC 73073..73741 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  CRG85_RS00345 (CRG85_00345) rpmB 73958..74194 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CRG85_RS00350 (CRG85_00350) rpmG 74215..74382 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CRG85_RS00355 (CRG85_00355) mutM 74480..75289 (+) 810 WP_001114529.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CRG85_RS00360 (CRG85_00360) coaD 75328..75807 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  CRG85_RS00365 (CRG85_00365) waaA 75815..77092 (-) 1278 WP_000891566.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CRG85_RS00370 (CRG85_00370) rfaQ 77499..78569 (+) 1071 WP_001311221.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=290726 CRG85_RS00340 WP_001298959.1 73073..73741(+) (radC) [Escherichia coli strain ECCNB20-2]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=290726 CRG85_RS00340 WP_001298959.1 73073..73741(+) (radC) [Escherichia coli strain ECCNB20-2]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGTTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496