Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   ENE68_RS00360 Genome accession   NZ_CP034784
Coordinates   78719..79387 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain ECZP248     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 73719..84387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ENE68_RS00330 (ENE68_00330) yicC 73738..74601 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  ENE68_RS00335 (ENE68_00335) rph 74728..75444 (+) 717 WP_001247089.1 ribonuclease PH -
  ENE68_RS00340 (ENE68_00340) pyrE 75510..76151 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  ENE68_RS00345 (ENE68_00345) slmA 76188..76784 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  ENE68_RS00350 (ENE68_00350) dut 76891..77349 (-) 459 WP_000976070.1 dUTP diphosphatase -
  ENE68_RS00355 (ENE68_00355) coaBC 77327..78547 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  ENE68_RS00360 (ENE68_00360) radC 78719..79387 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  ENE68_RS00365 (ENE68_00365) rpmB 79604..79840 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  ENE68_RS00370 (ENE68_00370) rpmG 79861..80028 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  ENE68_RS00375 (ENE68_00375) mutM 80126..80935 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  ENE68_RS00380 (ENE68_00380) coaD 80974..81453 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  ENE68_RS00385 (ENE68_00385) waaA 81461..82738 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  ENE68_RS00390 (ENE68_00390) rfaQ 83151..84209 (+) 1059 WP_000360406.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=290651 ENE68_RS00360 WP_001297375.1 78719..79387(+) (radC) [Escherichia coli strain ECZP248]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=290651 ENE68_RS00360 WP_001297375.1 78719..79387(+) (radC) [Escherichia coli strain ECZP248]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCATCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496