Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EM854_RS15030 Genome accession   NZ_CP034738
Coordinates   3051614..3052282 (-) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain L65     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3046614..3057282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EM854_RS15000 (EM854_15000) rfaQ 3046792..3047850 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  EM854_RS15005 (EM854_15005) waaA 3048263..3049540 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EM854_RS15010 (EM854_15010) coaD 3049548..3050027 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  EM854_RS15015 (EM854_15015) mutM 3050066..3050875 (-) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EM854_RS15020 (EM854_15020) rpmG 3050973..3051140 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  EM854_RS15025 (EM854_15025) rpmB 3051161..3051397 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  EM854_RS15030 (EM854_15030) radC 3051614..3052282 (-) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  EM854_RS15035 (EM854_15035) coaBC 3052454..3053674 (+) 1221 WP_000050115.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EM854_RS15040 (EM854_15040) dut 3053652..3054110 (+) 459 WP_000976070.1 dUTP diphosphatase -
  EM854_RS15045 (EM854_15045) slmA 3054217..3054813 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EM854_RS15050 (EM854_15050) pyrE 3054850..3055491 (-) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  EM854_RS15055 (EM854_15055) rph 3055557..3056273 (-) 717 WP_001247093.1 ribonuclease PH -
  EM854_RS15060 (EM854_15060) yicC 3056400..3057263 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=290616 EM854_RS15030 WP_001298959.1 3051614..3052282(-) (radC) [Escherichia coli strain L65]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=290616 EM854_RS15030 WP_001298959.1 3051614..3052282(-) (radC) [Escherichia coli strain L65]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment