Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   EM854_RS06280 Genome accession   NZ_CP034738
Coordinates   1253226..1254653 (+) Length   475 a.a.
NCBI ID   WP_000980607.1    Uniprot ID   -
Organism   Escherichia coli strain L65     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1248226..1259653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EM854_RS06250 (EM854_06250) cobU 1248498..1249043 (-) 546 WP_000973170.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -
  EM854_RS06255 (EM854_06255) - 1249210..1249401 (+) 192 Protein_1228 EutP/PduV family microcompartment system protein -
  EM854_RS06260 (EM854_06260) tmaR 1249502..1249831 (-) 330 WP_000450409.1 DUF496 family protein -
  EM854_RS06265 (EM854_06265) yeeA 1250003..1251061 (-) 1059 WP_001200891.1 FUSC family protein -
  EM854_RS06270 (EM854_06270) sbmC 1251259..1251732 (-) 474 WP_001105410.1 DNA gyrase inhibitor SbmC -
  EM854_RS06275 (EM854_06275) dacD 1251851..1253017 (-) 1167 WP_000830169.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  EM854_RS06280 (EM854_06280) sbcB 1253226..1254653 (+) 1428 WP_000980607.1 exodeoxyribonuclease I Machinery gene
  EM854_RS06285 (EM854_06285) tsuB 1254696..1254923 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  EM854_RS06290 (EM854_06290) tsuA 1254937..1255995 (-) 1059 WP_000492344.1 thiosulfate utilization transporter TsuA/YeeE -
  EM854_RS06295 (EM854_06295) plaP 1256174..1257532 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  EM854_RS06300 (EM854_06300) yoeI 1257522..1257584 (-) 63 WP_010723108.1 membrane protein YoeI -
  EM854_RS06305 (EM854_06305) yeeY 1257799..1258728 (-) 930 Protein_1238 LysR substrate-binding domain-containing protein -
  EM854_RS06310 (EM854_06310) yeeZ 1258774..1259598 (-) 825 WP_000754749.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54458.73 Da        Isoelectric Point: 5.4700

>NTDB_id=290586 EM854_RS06280 WP_000980607.1 1253226..1254653(+) (sbcB) [Escherichia coli strain L65]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=290586 EM854_RS06280 WP_000980607.1 1253226..1254653(+) (sbcB) [Escherichia coli strain L65]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTGCTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATCGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTCGAT
AAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAGGGTTATGCAGATGAATTGCAGATGCTGGCACAACAAT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583

Multiple sequence alignment