Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   EKM58_RS01215 Genome accession   NZ_CP034589
Coordinates   239078..240907 (+) Length   609 a.a.
NCBI ID   WP_001395096.1    Uniprot ID   -
Organism   Escherichia coli strain L37     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 234078..245907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKM58_RS24035 ysgD 234340..234396 (+) 57 WP_211180520.1 protein YsgD -
  EKM58_RS01190 (EKM58_01190) corA 234548..235498 (+) 951 WP_000947159.1 magnesium/cobalt transporter CorA -
  EKM58_RS24165 - 235541..235693 (-) 153 Protein_224 hypothetical protein -
  EKM58_RS01195 (EKM58_01195) - 236152..236153 (-) 2 WP_001390281.1 IS1-like element IS1B family transposase -
  EKM58_RS01200 (EKM58_01200) rarD 236502..237392 (-) 891 WP_126675391.1 EamA family transporter RarD -
  EKM58_RS01205 (EKM58_01205) yigI 237444..237911 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  EKM58_RS01210 (EKM58_01210) pldA 238076..238945 (+) 870 WP_001259700.1 phospholipase A -
  EKM58_RS01215 (EKM58_01215) recQ 239078..240907 (+) 1830 WP_001395096.1 ATP-dependent DNA helicase RecQ Machinery gene
  EKM58_RS01220 (EKM58_01220) rhtC 240971..241591 (+) 621 WP_000928821.1 threonine export protein RhtC -
  EKM58_RS01225 (EKM58_01225) rhtB 241653..242273 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  EKM58_RS01230 (EKM58_01230) pldB 242384..243406 (+) 1023 WP_000487640.1 lysophospholipase L2 -
  EKM58_RS01235 (EKM58_01235) yigL 243414..244214 (+) 801 WP_000285362.1 sugar/pyridoxal phosphate phosphatase YigL -
  EKM58_RS01240 (EKM58_01240) bioP 244290..245189 (+) 900 WP_063501904.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68373.55 Da        Isoelectric Point: 7.2544

>NTDB_id=290229 EKM58_RS01215 WP_001395096.1 239078..240907(+) (recQ) [Escherichia coli strain L37]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKPGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=290229 EKM58_RS01215 WP_001395096.1 239078..240907(+) (recQ) [Escherichia coli strain L37]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACCGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
GATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGCCCGGCATT
ATCTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGTATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTCCGCGCCGACGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCTGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGTAAACCGTTTATGGCGCTTATCCGCGCGCATGTTGACGGCGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391

Multiple sequence alignment