Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EKM58_RS00245 Genome accession   NZ_CP034589
Coordinates   43249..43917 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain L37     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 38249..48917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKM58_RS00215 (EKM58_00215) rfaQ 38427..39485 (-) 1059 WP_072666949.1 lipopolysaccharide core heptosyltransferase RfaQ -
  EKM58_RS00220 (EKM58_00220) waaA 39898..41175 (+) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EKM58_RS00225 (EKM58_00225) coaD 41183..41662 (+) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  EKM58_RS00230 (EKM58_00230) mutM 41701..42510 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EKM58_RS00235 (EKM58_00235) rpmG 42608..42775 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  EKM58_RS00240 (EKM58_00240) rpmB 42796..43032 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  EKM58_RS00245 (EKM58_00245) radC 43249..43917 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EKM58_RS00250 (EKM58_00250) coaBC 44089..45309 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EKM58_RS00255 (EKM58_00255) dut 45287..45745 (+) 459 WP_000976070.1 dUTP diphosphatase -
  EKM58_RS00260 (EKM58_00260) slmA 45852..46448 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EKM58_RS00265 (EKM58_00265) pyrE 46485..47126 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EKM58_RS00270 (EKM58_00270) rph 47192..47908 (-) 717 WP_001247089.1 ribonuclease PH -
  EKM58_RS00275 (EKM58_00275) yicC 48035..48898 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=290219 EKM58_RS00245 WP_001297375.1 43249..43917(-) (radC) [Escherichia coli strain L37]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=290219 EKM58_RS00245 WP_001297375.1 43249..43917(-) (radC) [Escherichia coli strain L37]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496